Patents by Inventor Kristine Bielecki

Kristine Bielecki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • COMPOSITIONS AND METHODS FOR PREPARING CAPPED MRNA
    Publication number: 20240060107
    Abstract: A method for in vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, and a recombinant RNA polymerase, and incubating the reaction mixture at from about 25° C. to about 40° C. for from about 1 hour to about 12 hours thereby producing the RNA. A method for in vitro transcription includes providing a DNA template and a cap analogue that binds to ?1 and/or +1 nucleotides of promoter for in vitro transcription, thus producing more full length mRNAs, allowing for more flexibility on the choice of first mRNA base, and providing +2 position open for custom sequence.
    Type: Application
    Filed: December 20, 2022
    Publication date: February 22, 2024
    Inventors: Cheng-Hsien WU, Fengmei PI, Robert DEMPCY, Shambhavi SHUBHAM, Kristine BIELECKI, Aaron BALL
  • Compositions and methods for preparing capped mRNA
    Patent number: 11898186
    Abstract: A method for in vitro transcription of a DNA template into RNA includes providing a mixture containing a buffer substance, ribonucleoside triphosphates (NTPs), one or more magnesium salts in a concentration of from about 2 mM to about 60 mM, the DNA template, and a recombinant RNA polymerase, and incubating the reaction mixture at from about 25° C. to about 40° C. for from about 1 hour to about 12 hours thereby producing the RNA. A method for in vitro transcription includes providing a DNA template and a cap analogue that binds to ?1 and/or +1 nucleotides of promoter for in vitro transcription, thus producing more full length mRNAs, allowing for more flexibility on the choice of first mRNA base, and providing +2 position open for custom sequence.
    Type: Grant
    Filed: December 20, 2022
    Date of Patent: February 13, 2024
    Assignee: GENSCRIPT USA INC.
    Inventors: Cheng-Hsien Wu, Fengmei Pi, Robert Dempcy, Shambhavi Shubham, Kristine Bielecki, Aaron Ball