Patents by Inventor Kristine Holley
Kristine Holley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8529889Abstract: The invention relates to compositions and methods for the modulation of the permeability of the epithelial cell barrier complex. In particular, the invention provides compositions and methods for using polysaccharides, preferably glycosaminoglycans, and agents that modify cell surface glycosaminoglycans, preferably glycosaminoglycan-degrading enzymes to modulate intercellular junctions. The compositions and methods provided can be used to facilitate the delivery of biologically active molecules.Type: GrantFiled: June 29, 2005Date of Patent: September 10, 2013Assignee: Massachusetts Institute of TechnologyInventors: Aarthi Chandrasekaran, Shiladitya Sengupta, David A. Berry, Kristine Holley, Ganlin Zhao, Ram Sasisekharan
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Publication number: 20120027744Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: March 30, 2011Publication date: February 2, 2012Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGYInventors: DONGFANG LIU, KEVIN POJASEK, ZACHARY SHRIVER, KRISTINE HOLLEY, YOSUF EL-SHABRAWI, GANESH VENKATARAMAN, RAM SASISEKHARAN
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Patent number: 7939292Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: GrantFiled: October 29, 2008Date of Patent: May 10, 2011Assignee: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20100119494Abstract: This invention relates, in part, to methods and compositions that modulate the stem cell environment. More specifically, the invention relates, in part, to methods and compositions for modulating stem cell differentiation. Therefore, methods and compositions are provided for modulating glycosaminoglycan moieties, e.g., heparan sulfate glycosaminoglycan (HSGAG) moieties, in the microenvironment of stem cells. Methods and compositions for promoting or inhibiting embryonic stem cell differentiation (e.g., differentiation into endothelial cells) are also provided. This invention also relates, therefore, in part, to cell populations (e.g., endothelial cell populations or impoverished endothelial cell populations) that can be produced with the methods and compositions provided. Furthermore, the invention relates, in part, to tissues, and uses thereof, formed by the methods and compositions provided. Moreover, the invention also relates, in part, to methods of treatment using the methods and compositions provided.Type: ApplicationFiled: January 19, 2010Publication date: May 13, 2010Applicant: Massachusetts Institute of TechnologyInventors: SHILADITYA SENGUPTA, RAM SASISEKHARAN, NISHLA KEISER, DAVID EAVARONE, TANYEL KIZILTEPE BILGICER, AARTHI CHANDRASEKARAN, DAVID A. BERRY, KRISTINE HOLLEY
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Publication number: 20090081635Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: October 29, 2008Publication date: March 26, 2009Applicant: Massachusetts Institute of TechnologyInventors: DONGFANG LIU, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Patent number: 7455986Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis , etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: GrantFiled: July 22, 2005Date of Patent: November 25, 2008Assignee: Massachusetts Institute of Technology, Inc.Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Patent number: 7390633Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: GrantFiled: April 18, 2006Date of Patent: June 24, 2008Assignee: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20070020243Abstract: This invention relates, in part, to methods and compositions that modulate the stem cell environment. More specifically, the invention relates, in part, to methods and compositions for modulating stem cell differentiation. Such modulation, in some aspects of the invention, is accomplished by agents that modulate glycosaminoglycans in the stem cell microenvironment (i.e., at or on the cell surface and/or in the extracellular matrix). Therefore, methods and compositions are provide for modulating glycosaminoglycan moieties, e.g., heparan sulfate glycosaminoglycan (HSGAG) moieties, in the microenvironment of stem cells. Methods and compositions for promoting or inhibiting embryonic stem cell differentiation (e.g., differentiation into endothelial cells) are also provided. This invention also relates, therefore, in part, to cell populations (e.g., endothelial cell populations or impoverished endothelial cell populations) that can be produced with the methods and compositions provided.Type: ApplicationFiled: January 12, 2006Publication date: January 25, 2007Applicant: Massachusetts Institute of TechnologyInventors: Shiladitya Sengupta, Ram Sasisekharan, Nishla Keiser, David Eavarone, Tanyel Kiziltepe Bilgicer, Aarthi Chandrasekaran, David Berry, Kristine Holley
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Publication number: 20060182734Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: April 18, 2006Publication date: August 17, 2006Applicant: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20060183713Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: April 18, 2006Publication date: August 17, 2006Applicant: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20060067927Abstract: The invention relates to compositions and methods for the modulation of the permeability of the epithelial cell barrier complex. In particular, the invention provides compositions and methods for using polysaccharides, preferably glycosaminoglycans, and agents that modify cell surface glycosaminoglycans, preferably glycosaminoglycan-degrading enzymes to modulate intercellular junctions. The compositions and methods provided can be used to facilitate the delivery of biologically active molecules.Type: ApplicationFiled: June 29, 2005Publication date: March 30, 2006Applicant: Massachusetts Institute of TechnologyInventors: Aarthi Chandrasekaran, Shiladitya Sengupta, David Berry, Kristine Holley, Ganlin Zhao, Ram Sasisekharan
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Publication number: 20060067928Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis , etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: July 22, 2005Publication date: March 30, 2006Applicant: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20050233402Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: October 14, 2004Publication date: October 20, 2005Applicant: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Patent number: 6869789Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: GrantFiled: March 8, 2001Date of Patent: March 22, 2005Assignee: Massachusetts Institute of TechnologyInventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20030099628Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase II having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: November 8, 2002Publication date: May 29, 2003Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
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Publication number: 20020122793Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.Type: ApplicationFiled: March 8, 2001Publication date: September 5, 2002Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan