Patents by Inventor Kristine Holley

Kristine Holley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8529889
    Abstract: The invention relates to compositions and methods for the modulation of the permeability of the epithelial cell barrier complex. In particular, the invention provides compositions and methods for using polysaccharides, preferably glycosaminoglycans, and agents that modify cell surface glycosaminoglycans, preferably glycosaminoglycan-degrading enzymes to modulate intercellular junctions. The compositions and methods provided can be used to facilitate the delivery of biologically active molecules.
    Type: Grant
    Filed: June 29, 2005
    Date of Patent: September 10, 2013
    Assignee: Massachusetts Institute of Technology
    Inventors: Aarthi Chandrasekaran, Shiladitya Sengupta, David A. Berry, Kristine Holley, Ganlin Zhao, Ram Sasisekharan
  • Publication number: 20120027744
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: March 30, 2011
    Publication date: February 2, 2012
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: DONGFANG LIU, KEVIN POJASEK, ZACHARY SHRIVER, KRISTINE HOLLEY, YOSUF EL-SHABRAWI, GANESH VENKATARAMAN, RAM SASISEKHARAN
  • Patent number: 7939292
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Grant
    Filed: October 29, 2008
    Date of Patent: May 10, 2011
    Assignee: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20100119494
    Abstract: This invention relates, in part, to methods and compositions that modulate the stem cell environment. More specifically, the invention relates, in part, to methods and compositions for modulating stem cell differentiation. Therefore, methods and compositions are provided for modulating glycosaminoglycan moieties, e.g., heparan sulfate glycosaminoglycan (HSGAG) moieties, in the microenvironment of stem cells. Methods and compositions for promoting or inhibiting embryonic stem cell differentiation (e.g., differentiation into endothelial cells) are also provided. This invention also relates, therefore, in part, to cell populations (e.g., endothelial cell populations or impoverished endothelial cell populations) that can be produced with the methods and compositions provided. Furthermore, the invention relates, in part, to tissues, and uses thereof, formed by the methods and compositions provided. Moreover, the invention also relates, in part, to methods of treatment using the methods and compositions provided.
    Type: Application
    Filed: January 19, 2010
    Publication date: May 13, 2010
    Applicant: Massachusetts Institute of Technology
    Inventors: SHILADITYA SENGUPTA, RAM SASISEKHARAN, NISHLA KEISER, DAVID EAVARONE, TANYEL KIZILTEPE BILGICER, AARTHI CHANDRASEKARAN, DAVID A. BERRY, KRISTINE HOLLEY
  • Publication number: 20090081635
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: October 29, 2008
    Publication date: March 26, 2009
    Applicant: Massachusetts Institute of Technology
    Inventors: DONGFANG LIU, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Patent number: 7455986
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis , etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Grant
    Filed: July 22, 2005
    Date of Patent: November 25, 2008
    Assignee: Massachusetts Institute of Technology, Inc.
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Patent number: 7390633
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Grant
    Filed: April 18, 2006
    Date of Patent: June 24, 2008
    Assignee: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20070020243
    Abstract: This invention relates, in part, to methods and compositions that modulate the stem cell environment. More specifically, the invention relates, in part, to methods and compositions for modulating stem cell differentiation. Such modulation, in some aspects of the invention, is accomplished by agents that modulate glycosaminoglycans in the stem cell microenvironment (i.e., at or on the cell surface and/or in the extracellular matrix). Therefore, methods and compositions are provide for modulating glycosaminoglycan moieties, e.g., heparan sulfate glycosaminoglycan (HSGAG) moieties, in the microenvironment of stem cells. Methods and compositions for promoting or inhibiting embryonic stem cell differentiation (e.g., differentiation into endothelial cells) are also provided. This invention also relates, therefore, in part, to cell populations (e.g., endothelial cell populations or impoverished endothelial cell populations) that can be produced with the methods and compositions provided.
    Type: Application
    Filed: January 12, 2006
    Publication date: January 25, 2007
    Applicant: Massachusetts Institute of Technology
    Inventors: Shiladitya Sengupta, Ram Sasisekharan, Nishla Keiser, David Eavarone, Tanyel Kiziltepe Bilgicer, Aarthi Chandrasekaran, David Berry, Kristine Holley
  • Publication number: 20060183713
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: April 18, 2006
    Publication date: August 17, 2006
    Applicant: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20060182734
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: April 18, 2006
    Publication date: August 17, 2006
    Applicant: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20060067927
    Abstract: The invention relates to compositions and methods for the modulation of the permeability of the epithelial cell barrier complex. In particular, the invention provides compositions and methods for using polysaccharides, preferably glycosaminoglycans, and agents that modify cell surface glycosaminoglycans, preferably glycosaminoglycan-degrading enzymes to modulate intercellular junctions. The compositions and methods provided can be used to facilitate the delivery of biologically active molecules.
    Type: Application
    Filed: June 29, 2005
    Publication date: March 30, 2006
    Applicant: Massachusetts Institute of Technology
    Inventors: Aarthi Chandrasekaran, Shiladitya Sengupta, David Berry, Kristine Holley, Ganlin Zhao, Ram Sasisekharan
  • Publication number: 20060067928
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis , etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: July 22, 2005
    Publication date: March 30, 2006
    Applicant: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20050233402
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: October 14, 2004
    Publication date: October 20, 2005
    Applicant: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Patent number: 6869789
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Grant
    Filed: March 8, 2001
    Date of Patent: March 22, 2005
    Assignee: Massachusetts Institute of Technology
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20030099628
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase II having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: November 8, 2002
    Publication date: May 29, 2003
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan
  • Publication number: 20020122793
    Abstract: The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
    Type: Application
    Filed: March 8, 2001
    Publication date: September 5, 2002
    Inventors: Dongfang Liu, Kevin Pojasek, Zachary Shriver, Kristine Holley, Yosuf El-Shabrawi, Ganesh Venkataraman, Ram Sasisekharan