Abstract: The invention relates to a non-invasive, simple, inexpensive, quick method to assess embryo competence in vitro by detecting nucleic acid-containing extracellular vesicles in the culture medium of the embryo. The culture medium may be fixed before being stained with propidium iodide and analysed by flow cytometry.

Abstract: A device and method for protecting cells, gametes, embryos, and oocytes from the harmful effect of illumination. The device is a culture dish that is optimized to create a dark environment inside of the dish. The culture dish features a base, an exterior wall connected to the outer rim of the base that forms an enclosure, and a lid connected to the exterior wall. The dish is preferably made of polystyrene and lid is preferably made of aluminum foil. The method features the following steps: reducing a room exposure to sunlight, where the cells and embryos are located; covering the room ceiling lighting with color filter-luminaires; shielding the transparent surfaces of the applied aspiration set and test tube from light; applying a colored red-light filter to the IVF workstation; and incubate cells and embryos in a light-protected place.

Abstract: The invention relates to a non-invasive, simple, inexpensive, quick method to assess embryo competence in vitro by detecting nucleic acid-containing extracellular vesicles in the culture medium of the embryo. The culture medium may be fixed before being stained with propidium iodide and analysed by flow cytometry.