Abstract: The present invention relates to a gesture sensing system that uses characteristic point as a positioning starting point to generate coordinate information in space for a test object. The gesture sensing system includes: a light emitter, a light sensor, and a signal processing module. The light emitter emits a plurality of emitted lights to the characteristic point and the test object, and the emitted light is reflected to generate a plurality of reflection light to be received and converted by the light sensor receives into a plurality of sensing signal. Then, the signal processing module generates initial coordinate information and movement coordinate information based on the sensing signals. Finally, the signal processing module generates a gesture according to the initial coordinate information and the change between the movement coordinate information, and executes a preset function according to the movement trajectory of the gesture.