Abstract: A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

Abstract: A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

Abstract: An optical sectioning apparatus including: a first white light source unit for generating a first white light beam; a light mixing unit facing the first white light source unit for dividing the first white light beam into a first partial beam and a second partial beam; a reference end unit for making the second partial beam travel a round trip along an adjustable optical path; a first objective lens having a collimated side facing the light mixing unit; a carrier unit facing a focal side of the first objective lens; a projection lens having a light entering side facing the light mixing unit; and a sensing unit facing a light exiting side of the projection lens.

Abstract: A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

Abstract: An optical sectioning apparatus combining Mirau optical interference microscopy and fluorescence microscopy, including: a wide band light source; an optical circulator, facing the wide band light source for splitting an incident light beam into a reflected light beam and a transmitted light beam; a short wavelength light source; a first dichroic splitter, facing the short wavelength light source and the optical circulator respectively, and being capable of providing a light-blocking effect on a band of wavelengths shorter than a preset wavelength; a Mirau interference objective lens, having a collimated side facing the first dichroic splitter; a sample carrier unit facing a focal side of the Mirau interference objective lens; a projection lens, having a light entrance side facing the optical circulator; and a sensor unit facing a light exit side of the projection lens.

Abstract: A method for producing image of biological sample, including steps: inputting a grayscale reflection image or a grayscale interference image of a biological sample into a first memory block; inputting a grayscale fluorescent image of the biological sample into a second memory block; using an information processing apparatus to convert the grayscale reflection image or the grayscale interference image into an RGB reflection image or an RGB interference image via first color transform operation and using the information processing apparatus to convert the grayscale fluorescent image into an RGB fluorescent image via second color transform operation; using the information processing apparatus to perform an image fusion operation and an intensity inversion operation on the RGB reflection image and the RGB fluorescent image or on the RGB interference image and the RGB fluorescent image to generate a pseudo H&E image; and outputting the pseudo H&E image to a display unit.

Abstract: An optical sectioning apparatus combining Mirau optical interference microscopy and fluorescence microscopy, including: a wide band light source; an optical circulator, facing the wide band light source for splitting an incident light beam into a reflected light beam and a transmitted light beam; a short wavelength light source; a first dichroic splitter, facing the short wavelength light source and the optical circulator respectively, and being capable of providing a light-blocking effect on a band of wavelengths shorter than a preset wavelength; a Mirau interference objective lens, having a collimated side facing the first dichroic splitter; a sample carrier unit facing a focal side of the Mirau interference objective lens; a projection lens, having a light entrance side facing the optical circulator; and a sensor unit facing a light exit side of the projection lens.

Abstract: A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

Abstract: An optical sectioning apparatus using optical interference microscopy and fluorescence microscopy, including: a beam splitter capable of splitting an incident light beam into a reflected light beam and a transmitted light beam; a wide band light source for providing the incident light beam; a reference arm unit for making the transmitted light beam travel a round trip along an adjustable optical path; a short wavelength light source; a first dichroic splitter, with a first side facing the short wavelength light source and a third side facing the beam splitter, being capable of providing a light-blocking effect on wavelengths shorter than a preset wavelength; an objective lens, with a collimated side facing a second side of the first dichroic splitter; a sample carrier unit facing a focal side of the objective lens; and a projection lens and a sensor units for receiving an output light beam.

Abstract: An optical sectioning apparatus using optical interference microscopy and fluorescence microscopy, including: a beam splitter capable of splitting an incident light beam into a reflected light beam and a transmitted light beam; a wide band light source for providing the incident light beam; a reference arm unit for making the transmitted light beam travel a round trip along an adjustable optical path; a short wavelength light source; a first dichroic splitter, with a first side facing the short wavelength light source and a third side facing the beam splitter, being capable of providing a light-blocking effect on wavelengths shorter than a preset wavelength; an objective lens, with a collimated side facing a second side of the first dichroic splitter; a sample carrier unit facing a focal side of the objective lens; and a projection lens and a sensor units for receiving an output light beam.

Abstract: An optical apparatus applied to ophthalmology detection is disclosed. The optical apparatus includes a first light source module, a second light source module, and an interference module. The first light source module is formed by a laser light source and lens units and used to emit a first light signal. The second light source module is formed by fiber units and lens units. The second light source module is coupled to the first light source module in series. The second light source module is used to receive a first light signal and emit a second light signal. The interference module is coupled to the second light source module and used to receive the second light signal and provide a first incident light and a second incident light to an object to be detected and a reference mirror respectively.

Abstract: A light source module of an optical apparatus is disclosed. The light source module includes a laser pump unit, a lens unit, and a fiber unit. The laser pump unit generates a laser source. The lens unit converts the laser source into a condensed beam. The fiber unit receives the condensed beam and emits an optical signal. The light source module can achieve effects of low cost, large bandwidth, high resolution, and high stability with well-designed pump power of the laser pump unit, and length, doping material, and core size of the fiber unit.

Abstract: The present invention relates to a manufacturing method of a double cladding crystal fiber, in which growing an YAG or a sapphire into a single crystal fiber by LHPG method, placing the single crystal fiber into a glass capillary for inner cladding, placing the single crystal fiber together with the glass capillary for inner cladding into a glass capillary for outer cladding in unison, heating the glass capillary for inner cladding and outer cladding by the LHPG method to attach to the outside of the single crystal fiber, and thus growing into a double cladding crystal fiber. When the present invention is applied to high power laser, by using the cladding pumping scheme, the high power pumping laser is coupled to the inner cladding layer, so the problems of heat dissipation and the efficiency impairment due to energy transfer up-conversion of high power laser are mitigated.

Abstract: A method for measuring characteristics of a sample is provided. The method includes the following steps: obtaining an interference spectrum of the sample; transforming the interference spectrum into a temporal interference signal via a Fourier transform, in which the temporal interference signal includes a plurality of coherence wave packets; separating the wave packets; transforming the wave packets into a plurality of interface interference signals via an inverse Fourier transform; and fitting a plurality of factors of the interface interference signals into a model for obtaining the refractive indexes, the extinction coefficients, and a thickness of the sample.

Abstract: The present invention relates to a crystal fiber, and more particularly to a Ti: sapphire crystal fiber, a manufacturing method thereof, and a wide band light source with the same. The Ti: sapphire single crystal is grown by means of laser-heated pedestal growth (LHPG) method into a crystal fiber of a predetermined diameter. The as-grown crystal fiber is annealed for enhancing its fluorescence and reducing the infra-red residual absorption. The annealed crystal fiber is inserted into a glass capillary and is grown into a single-clad crystal fiber. The wide band light source comprises: a pumping source for providing a pumping light; a single-clad Ti: sapphire crystal fiber for absorbing the pumping light and emitting the wide band light.

Abstract: An optical device for corneal measuring includes a light source module, a first optical module, a second optical module including a reference mirror, a light splitter and an image analysis unit. The light of the light source module is transmitted to the first and second optical modules through the light splitter. The light is transmitted to a cornea through the light splitter and the first optical module and reflected by the cornea to form a first light, the light is transmitted to the reference mirror through the light splitter and reflected by the reference mirror to form a second light. The first and second lights are transmitted to the light splitter and the image analysis unit. The reference mirror moves along a first direction, and when the first light and the second light interfere with each other, a relative optical path length is obtained.

Abstract: An optical device for corneal measuring includes a light source module, a first optical module, a second optical module including a reference mirror, a light splitter and an image analysis unit. The light of the light source module is transmitted to the first and second optical modules through the light splitter. The light is transmitted to a cornea through the light splitter and the first optical module and reflected by the cornea to form a first light, the light is transmitted to the reference mirror through the light splitter and reflected by the reference mirror to form a second light. The first and second lights are transmitted to the light splitter and the image analysis unit. The reference mirror moves along a first direction, and when the first light and the second light interfere with each other, a relative optical path length is obtained.

Abstract: The present invention relates to a manufacturing method of a double cladding crystal fiber, in which growing an YAG or a sapphire into a single crystal fiber by LHPG method, placing the single crystal fiber into a glass capillary for inner cladding, placing the single crystal fiber together with the glass capillary for inner cladding into a glass capillary for outer cladding in unison, heating the glass capillary for inner cladding and outer cladding by the LHPG method to attach to the outside of the single crystal fiber, and thus growing into a double cladding crystal fiber. When the present invention is applied to high power laser, by using the cladding pumping scheme, the high power pumping laser is coupled to the inner cladding layer, so the problems of heat dissipation and the efficiency impairment due to energy transfer up-conversion of high power laser are mitigated.

Abstract: The present invention relates to a double cladding crystal fiber and manufacturing method thereof, in which growing an YAG or a sapphire into a single crystal fiber by LHPG method, placing the single crystal fiber into a glass capillary for inner cladding, placing the single crystal fiber together with the glass capillary for inner cladding into a glass capillary for outer cladding in unison, heating the glass capillary for inner cladding and outer cladding by the LHPG method to attach to the outside of the single crystal fiber, and thus growing into a double cladding crystal fiber. When the present invention is applied to high power laser, by using the cladding pumping scheme, the high power pumping laser is coupled to the inner cladding layer, so the problems of heat dissipation and the efficiency impairment due to energy transfer up-conversion of high power laser are mitigated.

Abstract: The present invention relates to a crystal fiber, and more particularly to a Ti: sapphire crystal fiber, a manufacturing method thereof, and a wide band light source with the same. The Ti: sapphire single crystal is grown by means of laser-heated pedestal growth (LHPG) method into a crystal fiber of a predetermined diameter. The as-grown crystal fiber is annealed for enhancing its fluorescence and reducing the infra-red residual absorption. The annealed crystal fiber is inserted into a glass capillary and is grown into a single-clad crystal fiber. The wide band light source comprises: a pumping source for providing a pumping light; a single-clad Ti: sapphire crystal fiber for absorbing the pumping light and emitting the wide band light.