Abstract: The present disclosure provides a circCDK13-enriched engineered small extracellular vesicle (E-sEV), and a preparation method and use thereof, and belongs to the technical field of biomedicine. The present disclosure provides a preparation method of the circCDK13-enriched E-sEV. In the present disclosure, human placenta-derived mesenchymal stem cells (hP-MSCs) are infected with a vector overexpressing circCDK13, and a resulting cell supernatant is collected to obtain a small extracellular vesicle (sEV) overexpressing the circCDK13, which is used for wound healing in diabetes mellitus (DM). The E-sEV shows a therapeutic effect on DM wounds that is significantly better than that of natural small extracellular vesicles (N-sEVs) secreted by the hP-MSCs. This product not only exhibits advantages in healing speed, but also has a greater application potential in stimulating skin appendage regeneration and improving the quality of wound healing.

Abstract: The present disclosure belongs to the technical fields of genetic engineering and biotherapeutic drugs, and relates to an engineered exosome for treating hypertrophic scar (HTS), and a preparation method and use thereof. In the present disclosure, the engineered exosome for treating HTS is a mesenchymal stem cell (MSC)-derived exosome overexpressing miR-141-3p. The exosome can significantly reduce formation of the HTS.

Abstract: The present disclosure discloses an autophagosome for promoting healing of a diabetic wound, and a preparation method and use thereof. In the preparation method of the autophagosome provided in the present disclosure, the autophagosome is extracted from a starved vascular endothelial cell, and the production of the autophagosome does not require induction by an additional reagent. The preparation method of the present disclosure involves simple extraction conditions, does not require an ultracentrifuge, and can be implemented in an ordinary laboratory. In addition, a yield of the autophagosome is higher than a yield of an exosome. The present disclosure provides an easy-to-implement and low-cost treatment method without toxic and side effects for a patient suffering from a chronic refractory wound. The autophagosome of the present disclosure can promote healing of a diabetic wound, and reduce physiological and psychological burdens caused by a diabetic wound to a patient.

Abstract: The present disclosure provides a photocurable hydrogel loaded with VH298-modified exosome and a method of preparation and use thereof, belonging to the technical field of medical materials. In the present disclosure, an engineered exosome (VH-EVs) is prepared by combining an exosome with VH298, a hypoxia-inducible factor 1 alpha (HIF-1?) stabilizer; and a solid auxiliary material is loaded by a GelMA hydrogel. The material is not only conducive to sustained release of the engineered exosome, but improves angiogenesis and accelerate wound healing, showing a relatively high value for use.

Abstract: The present disclosure provides a photocurable hydrogel loaded with VH298-modified exosome and a method of preparation and use thereof, belonging to the technical field of medical materials. In the present disclosure, an engineered exosome (VH-EVs) is prepared by combining an exosome with VH298, a hypoxia-inducible factor 1 alpha (HIF-1?) stabilizer; and a solid auxiliary material is loaded by a GelMAhydrogel. The material is not only conducive to sustained release of the engineered exosome, but improves angiogenesis and accelerate wound healing, showing a relatively high value for use.

Abstract: A method and apparatus for recommending information are provided. The method includes: acquiring, in response to determining that a user enters a preset interface based on a first preset operation of the user, operation information within a preset length of time before the user performs the first preset operation; determining behavior data of the user browsing the preset interface based on the operation information; and determining recommendation information for the user based on the behavior data, and displaying the recommendation information in the preset interface.

Abstract: A control method of an electronic device system for diving is provided. The control method includes steps of sending a positioning request signal to the slave device; receiving a feedback signal sent by the slave device, the feedback signal is generated by the slave device responding to the positioning request signal, the feedback signal includes water pressure data; according to the time difference between the sending time of the positioning request signal and the receiving time of the feedback signal, and the signal transmission speed, the relative distance with the slave device is calculated; obtaining the relative direction between the slave device and the master device according to the incoming direction of the feedback signal received by the ultrasonic receiver; calculating the relative depth with the slave device according to the water pressure data in the feedback signal. Furthermore, an electronic device and system for diving are also provided.

Abstract: A control method of an electronic device system for diving is provided. The control method includes steps of sending a positioning request signal to the slave device; receiving a feedback signal sent by the slave device, the feedback signal is generated by the slave device responding to the positioning request signal, the feedback signal includes water pressure data; according to the time difference between the sending time of the positioning request signal and the receiving time of the feedback signal, and the signal transmission speed, the relative distance with the slave device is calculated; obtaining the relative direction between the slave device and the master device according to the incoming direction of the feedback signal received by the ultrasonic receiver; calculating the relative depth with the slave device according to the water pressure data in the feedback signal. Furthermore, an electronic device and system for diving are also provided.

Abstract: A linear constant-current LED drive circuit adaptive to a wide voltage range includes a rectifier bridge used for full-wave rectification of the waveform of a sinusoidal voltage and connected with a filter circuit and a high-voltage stabilizing and dropping circuit through wires, the filter circuit used for converting a full-wave pulsating voltage output by the rectifier bridge into a direct-current voltage and connected with a constant-current source circuit through a wire, the constant-current source circuit used for limiting a current across LED loads and providing a constant-current power supply for LED lights and connected with a switch array circuit through a wire, and the switch array circuit used for switching series-parallel connection modes of LED light strings by means of the switching characteristics of LDMODs when an external voltage varies. The high-voltage stabilizing and dropping circuit provides a working voltage for low-voltage modules.

Abstract: An item of jewellery, e.g. a ring, bracelet, bangle, watch, etc, has one or more magnets (1) in an annular arrangement (2). The magnetic pole(s) of one polarity (e.g. S) are distributed over at least a major sector, and preferably 360 degrees, of the annular arrangement at its radially-outward circumference, while the magnetic pole(s) of the opposite polarity (e.g. N) are presented at the radially-inward circumference of the annular to arrangement. The magnets are at least partially surrounded, but preferably completely enclosed, in decorative metal such as gold, silver, platinum, or their alloys. A perceptible attractive force may be established between a couple wearing a pair of the jewellery items in sufficiently close proximity and having complementary magnetic polarities, e.g. when one person wears a wedding ring having outwardly directed south poles, and the other person wears a wedding ring having outwardly directed north poles.