Abstract: The present invention provides ketoreductase variants having activity on both (R)-(?)-1,3-butanediol and (S)-(+)-1,3-butanediol, and the computational methodology for designing the above mentioned ketoreductase variants. The ketoreductase variants disclosed in the present application can catalyze the reaction of synthesizing 4-hydroxy-2-butanone from racemic 1,3-butanediol, which has industrial application prospects.

Abstract: A method for making intermediate compounds suitable for making muscenone, the compositions made by said methods, the various uses of said compositions, and enzyme variants useful in said methods.

Abstract: The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce ?-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing ?-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or ?-alanine. The use of the engineered polypeptides of the present invention for the preparation of ?-alanine results in higher unit activity, lower cost, and has good industrial application prospects.

Abstract: The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce ?-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing ?-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or ?-alanine. The use of the engineered polypeptides of the present invention for the preparation of ?-alanine results in higher unit activity, lower cost, and has good industrial application prospects.