Abstract: This invention describes a method of simulating an optical disk drive by a memory card, which is applied to a computer. The computer includes an operating system platform, and the memory card executes an auto-run program on the operating system platform like an optical disk drive. The method comprises the steps of: obtaining the capacity of said memory card; producing an ISO 9600 file system data on said memory card; computing the data length of a sector of said ISO 9660 file system data; computing the data length of a FAT section; producing the file system data of a FAT; loading said file system data of said FAT to said FAT section; and loading said ISO 9660 file system data to said ISO 9660 section. The invention also describes a method of reading the memory card having an auto-run function, and a card reading device for reading the memory card.