Abstract: Provided are active agent delivery devices configured to deliver an active agent formulation into or through a mucosal layer in a subject. The active agent delivery devices include a power reservoir configured to eject the active agent formulation at a pressure sufficient to deliver the active agent formulation into or through a mucosal layer in a subject. Methods of using the subject active agent delivery devices are also provided.

Abstract: A refrigerator includes a cabinet including a space, a door configured to open and close the space and including a light source and a front plate, through which light emitted from the light source passes, an output interface provided on the cabinet or the door to output sound, a communication unit configured to communicate with a remote device and a controller configured to play music through the output interface and to control the light source in association with music playback through the output interface, when a music playback command is received from the remote device through the communication interface.

Abstract: A home appliance includes a cabinet having a space, a door configured to open and close the space and including a light source, an illuminance sensor provided in the cabinet or the door and configured to detect illuminance, a user detection sensor provided in the cabinet or the door and configured to detect user proximity, and a controller configured to control the light source based on information detected by the user detection sensor, wherein, when a user is detected to approach the home appliance within a reference distance by the user detection sensor, the controller controls the light source of the door to be changed from a first state to a second state, and the reference distance is changeable based on one or more of an illuminance and current time detected by the illuminance sensor.

Abstract: Provided are active agent delivery devices configured to deliver an active agent formulation into or through a mucosal layer in a subject. The active agent delivery devices include a power reservoir configured to eject the active agent formulation at a pressure sufficient to deliver the active agent formulation into or through a mucosal layer in a subject. Methods of using the subject active agent delivery devices are also provided.

Abstract: A home appliance includes a cabinet having a space, a door configured to open and close the space and including a light source, an illuminance sensor provided in the cabinet or the door and configured to detect illuminance, a user detection sensor provided in the cabinet or the door and configured to detect user proximity, and a controller configured to control the light source based on information detected by the user detection sensor, wherein, when a user is detected to approach the home appliance within a reference distance by the user detection sensor, the controller controls the light source of the door to be changed from a first state to a second state, and the reference distance is changeable based on one or more of an illuminance and current time detected by the illuminance sensor.

Abstract: A refrigerator includes a cabinet including a space, a door configured to open and close the space and including a light source and a front plate, through which light emitted from the light source passes, an output interface provided on the cabinet or the door to output sound, a communication unit configured to communicate with a remote device and a controller configured to play music through the output interface and to control the light source in association with music playback through the output interface, when a music playback command is received from the remote device through the communication interface.

Abstract: Provided is a polymer comprising a hydrolysable polymer backbone, the polymer backbone comprising (i) monomer units comprising a hydrophobic side chain; and (ii) monomer units comprising a cationic side chain; wherein the cationic side chain comprises a polyamine with at least one tertiary amine and only a single nucleophilic center, optionally at the terminus of the polyamine, as well as a method of preparing said polymer, and a method of delivering a nucleic acid and/or polypeptide to a cell using the polymer.

Abstract: A home appliance includes a cabinet having a space, a door configured to open and close the space and including a light source, an illuminance sensor provided in the cabinet or the door and configured to detect illuminance, a user detection sensor provided in the cabinet or the door and configured to detect user proximity, and a controller configured to control the light source based on information detected by the user detection sensor, wherein, when a user is detected to approach the home appliance within a reference distance by the user detection sensor, the controller controls the light source of the door to be changed from a first state to a second state, and the reference distance is changeable based on one or more of an illuminance and current time detected by the illuminance sensor.

Abstract: Provided is a polymer comprising a hydrolysable polymer backbone, the polymer backbone comprising (i) monomer units with a side chain comprising a hydrophobic group; (ii) monomer units with a side chain comprising an oligoamine or polyamine; and optionally (iii) monomer units with a side chain comprising an ionizable group, as well as a method of preparing said polymer, and a method of delivering a nucleic acid and/or polypeptide to a cell using the polymer.

Abstract: Provided is a polymer comprising a hydrolysable polymer backbone, the polymer backbone comprising (i) monomer units with a side chain comprising a hydrophobic group; (ii) monomer units with a side chain comprising an oligoamine or polyamine; and (iii) monomer units with a side chain comprising a polyalkylene oxide, polyglycolic acid, polylactic acid, or combination thereof, as well as a composition comprising the polymer, and a method of delivering one or more biomolecules or synthetic variants thereof to a cell using the polymer and/or the composition.

Abstract: The invention provides a modified guide RNA comprising an extension sequence, as well as related compositions and methods.

Abstract: Provided is a polymer comprising a structure of Formula 1 or Formula 3 as provided herein, as well as a method of making the same; a composition comprising the polymer and a nucleic acid and/or polypeptide; and a method of delivering a nucleic acid and/or polypeptide to a cell.

Abstract: Provided is a polymer comprising a structure of Formula (1): (1) and a method of preparing said polymer. Also provided is a composition comprising the polymer and a nucleic acid and/or polypeptide, and a method of delivering a nucleic acid and/or polypeptide to a cell.

Abstract: Described herein are compositions and methods for treating diseases and disorders of the brain using a non-viral nanoparticle delivery of CRISPR. Disclosed herein are compositions comprising CRISPR-Gold compositions comprising DNA oligonucleotides, RNA-directed nucleases and guide RNAs. The methods include modulating expression of a gene in a cell using said compositions, inducing site-specific DNA cleavage in a cell, and treating a subject having fragile X syndrome caused by increased metabotropic glutamate receptor 5 signaling using the compositions disclosed herein.

Abstract: The present disclosure provides methods and compositions utilizing CRISPR systems wherein the guide RNA and the donor polynucleotide are modified.

Abstract: Provided are active agent delivery devices configured to deliver an active agent formulation into or through a mucosal layer in a subject. The active agent delivery devices include a power reservoir configured to eject the active agent formulation at a pressure sufficient to deliver the active agent formulation into or through a mucosal layer in a subject. Methods of using the subject active agent delivery devices are also provided.

Abstract: The invention provides a nucleic acid comprising a Cpf1 crRNA, a processing sequence 5? of the Cpf1 crRNA, and an extension sequence 5? of the processing sequence. The invention also provides a composition comprising the nucleic acid, a carrier, and optionally Cpf1. Additionally, the invention provides method of genetically modifying a eukaryotic target cell, comprising contacting the eukaryotic target cell with the nucleic acid or the composition to genetically modify a target nucleic acid in the cell.

Abstract: Provided are active agent delivery devices configured to deliver an active agent formulation into or through a mucosal layer in a subject. The active agent delivery devices include a power reservoir configured to eject the active agent formulation at a pressure sufficient to deliver the active agent formulation into or through a mucosal layer in a subject. Methods of using the subject active agent delivery devices are also provided.

Abstract: The present disclosure provides methods and compositions utilizing CRISPR systems wherein the guide RNA and the donor polynucleotide are modified.

Abstract: The present invention relates to a lithium secondary battery, and the lithium secondary battery comprises: a positive electrode comprising a positive active material represented by Chemical Formula 1, a plate-like conductive agent, and a spherical conductive agent; a negative electrode comprising a negative active material; and an electrolyte, wherein the positive electrode has a W value defined by Equation 1 of 0.9 to 2, LiaNixCoyMezO2??[Chemical Formula 1] wherein, in Chemical Formula 1, 0.9?a?1.1, 0.5?x?0.90, 0.05?y?0.5, 0.01?z?0.5, x+y+z=1, and Me is Mn or Al.