Abstract: A firewall manager automates traffic route configuration, compliance monitoring, and remediation. An administrator specifies a firewall policy that includes rules and traffic description, and specifies accounts, isolated virtual networks (IVNs) and/or subnets for firewall deployment. For automated traffic route configuration, the manager provisions and configures firewalls for the specified networks. The manager uses discovered network resource of the specified networks to determine route information for the firewalls for the networks for the traffic, and sends instructions for routing updates for the IVNs to an IVN manager service. For compliance monitoring and remediation, the manager obtains information about new IVNs, subnets, resources and/or routes, and determines compliance by comparing the obtained information to the firewall policy. The manager generates a remediation plan for non-compliance and instructs remediation by performing programmatic calls to the IVN manager service.

Abstract: The present invention relates generally to the use of a class of surfactants for emulsion and droplet polymerase chain reaction (“PCR”) mixtures. The class of surfactants consists of those having the chemical formula R—(OCH2CH2)n—OH, wherein R is an alkyl group consisting of 12 to 18 carbons and n is 2 to 25. The present invention also relates to methods, devices, systems, and kits incorporating the above-described class of surfactants.

Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.

Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.

Abstract: The present invention relates generally to the use of a class of surfactants for emulsion and droplet polymerase chain reaction (“PCR”) mixtures. The class of surfactants consists of those having the chemical formula R—(OCH2CH2)n—OH, wherein R is an alkyl group consisting of 12 to 18 carbons and n is 2 to 25. The present invention also relates to methods, devices, systems, and kits incorporating the above-described class of surfactants.

Abstract: The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.