Patents by Inventor Kunihisa Nagino
Kunihisa Nagino has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220372415Abstract: A method of biochip production includes a step A of applying a solution containing a selective binding substance, a salt, and a condensing agent to the surface of a substrate, a step B of immobilizing the selective binding substance to the surface of the substrate, a step C of drying the applied solution to allow the salt in the solution to precipitate on the surface of the substrate, and a step D of using an image detection device to detect a precipitate of the salt formed in the step C, wherein, in the step A, the condensing agent in the solution has a concentration of not less than 30 mM and not more than 80 mM.Type: ApplicationFiled: October 28, 2020Publication date: November 24, 2022Inventors: Yoichiro Koshi, Kunihisa Nagino
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Patent number: 9823197Abstract: A detecting method includes irradiating a substrate having a concave and convex shape, with laser light collected by a lens; and detecting a height difference of the concave and convex shape by acquiring light intensity of reflected light and/or scattered light from the substrate as image data, and a light irradiation surface of the substrate is arranged at a position closer to the lens than a focal position of the lens is, reflected light and/or scattered light from the light irradiation surface is received as detected light, and a height difference of the substrate is detected based on a change in intensity of the received light.Type: GrantFiled: October 4, 2013Date of Patent: November 21, 2017Assignee: Toray Industries, Inc.Inventors: Kunihisa Nagino, Syunpei Yoshikawa
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Patent number: 9719929Abstract: A microarray analysis method, in which a microarray obtained by arranging probes on a substrate surface having an irregular shape is irradiated with excitation light and fluorescence amounts of the probes excited by the excitation light are obtained as numerical data, includes a step (a) of measuring the fluorescence amounts of the probes to acquire fluorescence image data, a step (b) of receiving reflected light and/or scattered light from the substrate surface to acquire the irregular shape of the substrate surface of the microarray as alignment image data based on the light receiving intensities of the light, and a step (c) of determining positions of the probes on the fluorescence image data based on the alignment image data.Type: GrantFiled: December 26, 2011Date of Patent: August 1, 2017Assignee: Toray Industries, Inc.Inventors: Kumie Ozaki, Hiromichi Sasamoto, Kunihisa Nagino
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Patent number: 9519822Abstract: A determination method for determining reliability of a selective binding amount of a substance to be examined obtained as detection intensity of a label when a labeled substance to be examined binds to a selective binding substance fixed as a spot on a carrier includes: determining a position of the spot in image data obtained by imaging the detection intensity in the carrier and extracting a pixel group corresponding to the spot; calculating a ratio or a difference between a median value of the detection intensity of the pixel group extracted at the determining and a median value of the detection intensity of the pixel group excluding a certain top proportion of and/or a certain bottom proportion of pixels; and determining quality of the reliability based on the ratio or the difference calculated at the calculating and a certain reference value.Type: GrantFiled: March 5, 2013Date of Patent: December 13, 2016Assignee: Toray Industries, Inc.Inventors: Kunihisa Nagino, Hiromichi Sasamoto, Shigeru Suzue
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Patent number: 9358518Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.Type: GrantFiled: July 28, 2010Date of Patent: June 7, 2016Assignee: Toray Industries, Inc.Inventors: Kunihisa Nagino, Fumio Nakamura, Hitoshi Nobumasa
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Patent number: 9333478Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.Type: GrantFiled: July 28, 2010Date of Patent: May 10, 2016Assignee: Toray Industries, Inc.Inventors: Kunihisa Nagino, Fumio Nakamura, Hitoshi Nobumasa
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Publication number: 20150276604Abstract: A detecting method includes irradiating a substrate having a concave and convex shape, with laser light collected by a lens; and detecting a height difference of the concave and convex shape by acquiring light intensity of reflected light and/or scattered light from the substrate as image data, and a light irradiation surface of the substrate is arranged at a position closer to the lens than a focal position of the lens is, reflected light and/or scattered light from the light irradiation surface is received as detected light, and a height difference of the substrate is detected based on a change in intensity of the received light.Type: ApplicationFiled: October 4, 2013Publication date: October 1, 2015Inventors: Kunihisa Nagino, Syunpei Yoshikawa
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Publication number: 20150098611Abstract: A determination method for determining reliability of a selective binding amount of a substance to be examined obtained as detection intensity of a label when a labeled substance to be examined binds to a selective binding substance fixed as a spot on a carrier includes: determining a position of the spot in image data obtained by imaging the detection intensity in the carrier and extracting a pixel group corresponding to the spot; calculating a ratio or a difference between a median value of the detection intensity of the pixel group extracted at the determining and a median value of the detection intensity of the pixel group excluding a certain top proportion of and/or a certain bottom proportion of pixels; and determining quality of the reliability based on the ratio or the difference calculated at the calculating and a certain reference value.Type: ApplicationFiled: March 5, 2013Publication date: April 9, 2015Inventors: Kunihisa Nagino, Hiromichi Sasamoto, Shigeru Suzue
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Publication number: 20130303403Abstract: A microarray analysis method, in which a microarray obtained by arranging probes on a substrate surface having an irregular shape is irradiated with excitation light and fluorescence amounts of the probes excited by the excitation light are obtained as numerical data, includes a step (a) of measuring the fluorescence amounts of the probes to acquire fluorescence image data, a step (b) of receiving reflected light and/or scattered light from the substrate surface to acquire the irregular shape of the substrate surface of the microarray as alignment image data based on the light receiving intensities of the light, and a step (c) of determining positions of the probes on the fluorescence image data based on the alignment image data.Type: ApplicationFiled: December 26, 2011Publication date: November 14, 2013Applicant: Toray Industries, Inc.Inventors: Kumie Ozaki, Hiromichi Sasamoto, Kunihisa Nagino
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Patent number: 8298832Abstract: The method of stirring a solution according to the present invention is a method of stirring a solution comprising contacting a selective binding substance immobilized on the surface of a carrier with a solution containing an analyte substance reactive with the selective binding substance, and mixing the fine particles or air bubbles into the solution containing an analyte substance, and moving the fine particles or air bubbles without allowing contact thereof with the selective binding substance-immobilized surface. The present invention provides a stirring method that accelerates the reaction of a carrier-immobilized selective binding substance with an analyte substance and detects a trace amount of analyte at high signal intensity and high S/N ratio. The present invention enables diagnosis and examination in the clinical setting by using a selective binding substance-immobilized carrier such as DNA chip.Type: GrantFiled: March 17, 2005Date of Patent: October 30, 2012Assignee: Toray Industries, Inc.Inventors: Yuki Takii, Kunihisa Nagino, Fumio Nakamura, Hitoshi Nobumasa
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Publication number: 20110028347Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.Type: ApplicationFiled: July 28, 2010Publication date: February 3, 2011Applicant: TORAY INDUSTRIES, INC.Inventors: KUNIHISA NAGINO, FUMIO NAKAMURA, HITOSHI NOBUMASA
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Publication number: 20110015097Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.Type: ApplicationFiled: July 28, 2010Publication date: January 20, 2011Applicant: TORAY INDUSTRIES, INC.Inventors: KUNIHISA NAGINO, FUMIO NAKAMURA, HITOSHI NOBUMASA
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Patent number: 7795006Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.Type: GrantFiled: May 18, 2004Date of Patent: September 14, 2010Assignee: Toray Industries, Inc.Inventors: Kunihisa Nagino, Fumio Nakamura, Hitoshi Nobumasa
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Publication number: 20070178603Abstract: The method of stirring a solution according to the present invention is a method of stirring a solution comprising contacting a selective binding substance immobilized on the surface of a carrier with a solution containing an analyte substance reactive with the selective binding substance, and mixing the fine particles or air bubbles into the solution containing an analyte substance, and moving the fine particles or air bubbles without allowing contact thereof with the selective binding substance-immobilized surface. The present invention provides a stirring method that accelerates the reaction of a carrier-immobilized selective binding substance with an analyte substance and detects a trace amount of analyte at high signal intensity and high S/N ratio. The present invention enables diagnosis and examination in the clinical setting by using a selective binding substance-immobilized carrier such as DNA chip.Type: ApplicationFiled: March 17, 2005Publication date: August 2, 2007Inventors: Yuki Takii, Kunihisa Nagino, Fumio Nakamura, Hitoshi Nobumasa
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Publication number: 20070009958Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.Type: ApplicationFiled: May 18, 2004Publication date: January 11, 2007Applicant: Toray Industries, Inc.Inventors: Kunihisa Nagino, Fumio Nakamura, Hitoshi Nobumasa
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Publication number: 20040096169Abstract: Disclosed is an array immobilizing selective binding substances thereon, with which each sample can be distinguished without relying on the positions, an arbitrary array may be made, the density may be freely changed depending on the method of using, and with which the reaction between the bound sample and another sample can be detected easily. The present invention provides a fiber on which a selective-binding substance is immobilized, or a fiber array comprising a bundle of the fibers. A method for binding reaction between a selective binding substance and a corresponding selective binding substance, in which a test sample and/or the corresponding selective binding substance is(are) moved relative to the surface on which the selective binding substance is immobilized by, for example, applying an AC voltage in a direction crossing the perpendicular axis of the surface on which the selective binding substance is immobilized was also provided.Type: ApplicationFiled: December 11, 2003Publication date: May 20, 2004Inventors: Saburo Sone, Kunihisa Nagino, Masashi Higasa, Hitoshi Nobumasa, Koji Watanabe
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Patent number: 6352753Abstract: A rewritable phase change type optical recording medium having excellent in storage durability such as archival property and overwrite shelf property and is less likely to have increased jitters, lower contrast and bursting due to deterioration by repeated overwriting is disclosed. The optical recording medium comprises a first dielectric layer, a first boundary layer and a recording layer, in tis order, wherein the first boundary layer is in contact with the recording layer. The information in the optical recording medium can be recorded, erased and reproduced by irradiating the recording layer with light. The recording and erasure of information are affected by reversible phase change between amorphous phase and crystalline phase of the recording layer.Type: GrantFiled: November 3, 1999Date of Patent: March 5, 2002Assignee: Toray Industries, Inc.Inventors: Toshihisa Nonaka, Kunihisa Nagino, Takeshi Arai, Hideo Nakakuki
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Publication number: 20010048993Abstract: An object of the present invention is to provide a rewritable phase change type optical recording medium less likely to have increased jitters, lower contrast, bursting, etc. respectively caused as deterioration by repeated overwriting. Another object of the present invention is to provide a rewritable phase change type recording medium excellent in storage durability such as archival property and overwrite shelf property.Type: ApplicationFiled: November 3, 1999Publication date: December 6, 2001Inventors: TOSHIHISA NONAKA, KUNIHISA NAGINO, TAKESHI ARAI, HIDEO NAKAKUKI
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Patent number: 6300039Abstract: Phase-change type rewritable optical recording media, such as optical discs, optical cards, and optical tapes, where recorded data can be erased or overwritten and where data can be recorded with high speed and high density by applying a light beam can include an optical recording medium is one that includes a laminate member, which in various embodiments contains a transparent substrate, a first dielectric layer, a recording layer, a second dielectric layer, a light absorbing layer, and/or a reflecting layer. In various embodiments of the invention, the shortest distance between recorded marks in the recording direction along the track is less than &lgr;/NA, with &lgr; and NA denoting the wavelength of the light used and the numerical aperture of the objective lens of the optical head, respectively.Type: GrantFiled: October 16, 1997Date of Patent: October 9, 2001Assignee: Toray Industries, Inc.Inventors: Gentaro Ohbayashi, Hitoshi Nobumasa, Kusato Hirota, Kunihisa Nagino, Futoshi Okuyama
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Patent number: 6071588Abstract: An optical recording medium has a high hardness layer, first dielectric layer, recording layer, second dielectric layer and reflective layer. The hardness of the high hardness layer is larger than the hardness of the first dielectric layer, and the thickness of the second dielectric layer is in the range of 3 to 50 nm. Such a rewritable phase change type optical recording medium may show reduced degradation in the write-start portion and write-end portion of frequently rewritten sectors, and have good jitter characteristics.Type: GrantFiled: December 16, 1997Date of Patent: June 6, 2000Assignee: Toray Industries, Inc.Inventors: Hitoshi Nobumasa, Kunihisa Nagino, Gentaro Ohbay Ashi, Tomoya Yamashiki, Yuji Watanabe, Takeshi Arai