Patents by Inventor Kunio Isshiki
Kunio Isshiki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7235388Abstract: Provided are an isolated gene capable of participating in the production of L-homoglutamic acid, and a production system of L-homoglutamic acid by using this gene. The gene is derived from the genome of Flavobacterium lutescens.Type: GrantFiled: June 3, 2004Date of Patent: June 26, 2007Assignee: Mercian CorporationInventors: Tadashi Fujii, Takao Narita, Kuniho Nakata, Hitosi Agematu, Hiroshi Tsunekawa, Kunio Isshiki, Takeo Yoshioka
-
Patent number: 7060821Abstract: A compound represented by formula (I); a process for producing a compound (substance F-1490) of formula (I), wherein X represents —O— and R represents a hydroxyl group, by using a microorganism belonging to the genus Cunninghamella; and a Cunninghamella sp. F-1490 strain (FERM BP-8287) capable of producing the substance F-1490; and an osteoclast differentiation inhibitory agent containing as an active ingredient the compound represented by formula (I): wherein X represents —O— or —CH2—, and R represents a hydroxyl group when X represents —O—, or a hydrogen atom when X represents —CH2—.Type: GrantFiled: March 6, 2003Date of Patent: June 13, 2006Assignees: Mercian Corporation, Zaidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Hiroyuki Kumagai, Tomohiro Sameshima, Motoko Matsufuji, Naoto Kawamura, Kunio Isshiki, Hiroyuki Inoue, Tetsuya Someno, Masaaki Ishizuka, Tomio Takeuchi
-
Patent number: 7022502Abstract: A process for the production of L-pipecolic acid which comprises the step of reducing delta-1-piperideine-6-carboxylic acid by the use of pyrroline-5-carboxylate reductase. The delta-1-piperideine-6-carboxylic acid is obtained by the step of converting L-lysine by the use of lysine 6-aminotransferase encoded by a gene of Flavobacterium lutescens. The steps of reducing delta-1-piperideine-6-carboxylic acid and the converting of L-lysine into L-pipecolic acid by the use of lysine 6-aminotransferase are carried out by using a bacterium transformed with a gene encoding lysine 6-aminotransferase wherein such bacterium comprises pyrroline-5-carboxylate reductase encoded by a gene of Escherichia coli or a coryneform bacterium. A recombinant bacterium which can be used in this production process is also provided. Thus, the present invention can provide an efficient biological process for the production of L-pipecolic acid (or 2-piperidinecarboxylic acid).Type: GrantFiled: December 22, 2000Date of Patent: April 4, 2006Assignee: Mercian CorporationInventors: Tadashi Fujii, Yasuhide Aritoku, Manabu Mukaihara, Takao Narita, Hitosi Agematu, Kunio Isshiki
-
Publication number: 20050124050Abstract: A compound represented by formula (I); a process for producing a compound (substance F-1490) of formula (I), wherein X represents —O— and R represents a hydroxyl group, by using a microorganism belonging to the genus Cunninghamella; and a Cunninghamella sp. F-1490 strain (FERM BP-8287) capable of producing the substance F-1490; and an osteoclast differentiation inhibitory agent containing as an active ingredient the compound represented by formula (I): wherein X represents —O— or —CH2—, and R represents a hydroxyl group when X represents —O—, or a hydrogen atom when X represents —CH2—.Type: ApplicationFiled: March 6, 2003Publication date: June 9, 2005Applicant: Mercian Corporation and Zaidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Hiroyuki Kumagai, Tomohiro Sameshima, Motoko Matsufuji, Naoto Kawamura, Kunio Isshiki, Hiroyuki Inoue, Tetsuya Someno, Masaaki Sihizuka, Tomio Takeuchi
-
Patent number: 6890746Abstract: Provided are an isolated gene capable of participating in the production of L-homoglutamic acid, and a production system of L-homoglutamic acid by using this gene. The gene is derived from the genome of Flavobacterium lutescens.Type: GrantFiled: August 4, 1999Date of Patent: May 10, 2005Assignee: Mercian CorporationInventors: Tadashi Fujii, Takao Narita, Kuniho Nakata, Hitosi Agematu, Hiroshi Tsunekawa, Kunio Isshiki, Takeo Yoshioka
-
Publication number: 20040214295Abstract: Provided are an isolated gene capable of participating in the production of L-homoglutamic acid, and a production system of L-homoglutamic acid by using this gene. The gene is derived from the genome of Flavobacterium lutescens.Type: ApplicationFiled: June 3, 2004Publication date: October 28, 2004Inventors: Tadashi Fujii, Takao Narita, Kuniho Nakata, Hitosi Agematu, Hiroshi Tsunekawa, Kunio Isshiki, Takeo Yoshioka
-
Publication number: 20030207410Abstract: A process for the production of L-pipecolic acid which comprises the step of reducing delta-1-piperideine-6-carboxylic acid by the use of pyrroline-5-carboxylate reductase. A recombinant bacterium which can be used in this production process is also provided. Thus, the present invention can provide an efficient biological process for the production of L-pipecolic acid (or 2-piperidinecarboxylic acid).Type: ApplicationFiled: June 28, 2002Publication date: November 6, 2003Inventors: Tadashi Fujii, Yasuhide Aritoku, Manabu Mukaihara, Takao Narita, Hitosi Agematu, Kunio Isshiki
-
Patent number: 6566394Abstract: Salicylamide derivatives represented by formulae (1a) and (1b); intermediates in the production thereof; a process for producing the same; and drugs containing the same as the active ingredient. The salicylamide derivatives represented by formulae (1a) and (1b) are useful as anti-inflammatory agents and immunosuppressive agents which exert an effect of inhibiting the activation of NF-&kgr;B with little side effects.Type: GrantFiled: May 30, 2002Date of Patent: May 20, 2003Assignees: Mercian Corporation, Zaidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Tomio Takeuchi, Kazuo Umezawa, Sakino To-E, Naoki Matsumoto, Tsutomu Sawa, Takeo Yoshioka, Naoki Agata, Shin-ichi Hirano, Kunio Isshiki
-
Patent number: 6548695Abstract: Provided is a process for producing an isocoumarin-3-yl-acetic acid derivative, characterized by reacting a homophthalic acid derivative represented by a formula: (wherein Rc, R2 and R are mainly protecting groups) with a malonic acid derivating represented by a formula: (wherein R is an organic group including a lower alkyl group; R3 is a protecting group for a carboxyl group; and X is a halogen atom or a —OM group (wherein M is alkaline metal or alkaline earth metal)). According to the above process, various isocoumarin-3-yl-acetic acid derivatives can efficiently be provided.Type: GrantFiled: April 11, 2002Date of Patent: April 15, 2003Assignees: Mercian Corporation, Zaidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Toshio Tsuchida, Hazuki Nagai, Takashi Nakashima, Masashi Yoshida, Kaname Konuki, Asako Kuroda, Kunio Isshiki, Tomio Takeuchi
-
Publication number: 20020147350Abstract: Provided is a process for producing an isocoumarin-3-yl-acetic acid derivative, characterized by reacting a homophthalic acid derivative represented by a formula: 1Type: ApplicationFiled: April 11, 2002Publication date: October 10, 2002Inventors: Toshio Tsuchida, Hazuki Nagai, Takashi Nakashima, Masashi Yoshida, Kaname Konuki, Asako Kuroda, Kunio Isshiki, Tomio Takeuchi
-
Patent number: 6426420Abstract: Provided is a process for producing an isocoumarin-3-yl-acetic acid derivative, characterized by reacting a homophthalic acid derivative represented by a formula: (wherein Rc, R2 and R are mainly protecting groups) with a malonic acid derivating represented by a formula: (wherein R is an organic group including a lower alkyl group; R3 is a protecting group for a carboxyl group; and X is a halogen atom or a —OM group (wherein M is alkaline metal or alkaline earth metal)). According to the above process, various isocoumarin-3-yl-acetic acid derivatives can efficiently be provided.Type: GrantFiled: July 5, 2000Date of Patent: July 30, 2002Assignees: Mercian Corporation, Zaidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Toshio Tsuchida, Hazuki Nagai, Takashi Nakashima, Masashi Yoshida, Kaname Konuki, Asako Kuroda, Kunio Isshiki, Tomio Takeuchi
-
Patent number: 6361987Abstract: This invention provides DNA fragments for efficiently preparing a protein having asymmetric hydrolase activity for 4-substituted 1,4-dihydropyridine derivatives, transformants obtained by using such a DNA fragment, and a process for preparing the aforesaid enzyme. Specifically, an isolated gene derived from the chromosome of Streptomyces viridosporus and encoding a protein having asymmetric hydrolase activity for 4-substituted 1,4-dihydropyridine derivatives, plasmids having the aforesaid gene integrated thereinto, transformants obtained by using such a plasmid, and a process for preparing the aforesaid enzyme by using such a transformant are disclosed. A process for the enzymatic conversion of 4-substituted 1,4-dihydropyridine derivatives by using the aforesaid enzyme is also disclosed.Type: GrantFiled: February 28, 2000Date of Patent: March 26, 2002Assignee: Mercian CorporationInventors: Akira Arisawa, Motoko Matsufuji, Takurou Tsuruta, Kazuyuki Dobashi, Takashi Nakashima, Kunio Isshiki, Takeo Yoshioka
-
Patent number: 6143541Abstract: This invention provides DNA fragments for efficiently preparing a protein having asymmetric hydrolase activity for 4-substituted 1,4-dihydropyridine derivatives, transformants obtained by using such a DNA fragment, and a process for preparing the aforesaid enzyme. Specifically, an isolated gene derived from the chromosome of Streptomyces viridosporus and encoding a protein having asymmetric hydrolase activity for 4-substituted 1,4-dihydropyridine derivatives, plasmids having the aforesaid gene integrated thereinto, transformants obtained by using such a plasmid, and a process for preparing the aforesaid enzyme by using such a transformant are disclosed. A process for the enzymatic conversion of 4-substituted 1,4-dihydropyridine derivatives by using the aforesaid enzyme is also disclosed.Type: GrantFiled: January 30, 1998Date of Patent: November 7, 2000Assignee: Mercian CorporationInventors: Akira Arisawa, Motoko Matsufuji, Takurou Tsuruta, Kazuyuki Dobashi, Takashi Nakashima, Kunio Isshiki, Takeo Yoshioka
-
Patent number: 6133443Abstract: Optically active 1,4-dihydropyridine derivatives represented by general formula (I) and anion salts thereof and a production method therefor: ##STR1## (wherein R.sup.1 represents a C.sub.1-6 alkyl group, R.sup.2 represents a nitrogen-containing heterocyclic group forming a quaternary ammonium which may be substituted, and n is an integer of 1, 2 or 3). The optically active 1,4-dihydropyridine derivatives represented by general formula (I) have vasodilating activity and hypotensive activity and are excellent in water solubility so that they are useful as a drug for circulatory system such as a hypotensor or vasodilator.Type: GrantFiled: August 21, 1998Date of Patent: October 17, 2000Assignee: Mercian CorporationInventors: Takashi Nakashima, Kunio Isshiki, Noriaki Sakata, Naoki Agata, Takeo Yoshioka
-
Patent number: 5760238Abstract: Disclosed are 1,4-dihydropyridine derivatives represented by the general formula (I) and salts thereof, as well as optical active 1,4-dihydropyridine derivatives represented by the general formula (II) and salts thereof which are obtained from the derivative by biochemical reactions using microorganisms or enzymes and useful as preventive and therapeutic agents for ischemic heart diseases and hypertension. ##STR1## (wherein x and Y are H, NO.sub.2, CN, halogen; R.sup.1 and R.sup.2 are H, CHO, substituted alkylcarbonyl, crotonyl, cinnamoyl, alkoxycarbonyl, aryloxycarbonyl, benzyloxycarbonyl, carboxybenzoyl, or R.sup.1 and R.sup.2 taken together represent phthaloyl; n is an integer of 1 to 3).Type: GrantFiled: February 25, 1997Date of Patent: June 2, 1998Assignee: Mercian CorporationInventors: Kunio Isshiki, Naoki Matsumoto, Takashi Nakashima, Kazuyuki Dobashi, Takurou Tsuruta, Takeo Yoshioka
-
Patent number: 5635395Abstract: (4R)-3-(Substituted aminoalkyl)oxycarbonyl-1,4-dihydro-2,6-dimethyl-4-(nitrophenyl)pyridine-5- carboxylic acids can be prepared efficiently, by reacting bis(substituted aminoalkyl) 1,4-dihydro-2,6-dimethyl-4-(nitrophenyl)pyridine-3,5-dicarboxylates with a microorganism capable of asymmetric hydrolysis and belonging to the genus Streptomyces, the genus Paecilomyces, the genus Botryodioplodia, the genus Alternaria or the genus Helminthosporium, or a treated product thereof. The compounds are extremely useful as important intermediates for preparation of pharmaceuticals useful for the prevention and treatment of angina pectoris, hypertension, etc.Type: GrantFiled: February 21, 1995Date of Patent: June 3, 1997Assignees: Mercian Corporation, Taisho Pharmaceutical Co., Ltd.Inventors: Kunio Isshiki, Takashi Nakashima, Takeo Yoshioka, Hiroshi Tsunekawa, Takashi Adachi, Tomomi Ota
-
Patent number: 5395941Abstract: Optically active 1,4-dihydropyridine derivatives represented by general formula (I), process for preparing compounds (Ic) (in general formula (I) , R.sup.1 is a lower alkyl group), process for preparing compounds represented by general formula (II) by hydrolyzing the compounds (Ic) under acidic conditions, process for preparing compounds represented by general formula (II) by solvolysis, under basic conditions, of compounds represented by general formula (III); ##STR1## wherein R.sup.1 is H, a lower alkyl group, a cyanoethyl group, a lower alkanoylaminoethyl group, or a lower dialkylaminoethyl group; R.sup.2 is a 2-tetrahydropyranyl group or a lower alkyloxymethyl group; R.sup.3 is a cyano group, a lower dialkylamino group or a lower alkanoylamino group; and R.sup.4 is a lower alkyl group. The compounds of general formula (I) and (II) are intermediate compounds for preparing optically active 1,4-dihydropyridine derivatives which are useful as medicines for circulatory organs.Type: GrantFiled: March 22, 1994Date of Patent: March 7, 1995Assignee: Mercian CorporationInventors: Kunio Isshiki, Takashi Nakashima, Hiroshi Tanaka, Takeo Yoshioka
-
Patent number: 5098935Abstract: A new antibiotic, named Conagenin, is provided which can exhibit useful carcinostatic or antitumor activities on carcinomas and tumors, particularly malignant tumors, of mammalian animals including man. Conagenin can be produced by fermentation of microorganisms of genus Streptomyces, typically Streptomyces roseosporus MI696-AF3 strain identified under the deposit number FERM BP-2738. Conagenin has formula (I) and is useful for medicinal purposes, particularly as carcinostatic agent or antitumor agent for inhibiting carcinomas and malignant tumors of mammalian animals, including man.Type: GrantFiled: May 17, 1990Date of Patent: March 24, 1992Assignees: Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai, Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Masaaki Ishizuka, Takashi Yamashita, Hiroshi Naganawa, Hironobu Iinuma, Kunio Isshiki, Masa Hamada, Kenji Maeda, Tomio Takeuchi
-
Patent number: 5096924Abstract: As a new antibiotic is provided a compound, now nominated as MI43-37F11 substance, which has formula ##STR1## This MI43-37F11 substance has an antitumor activity, an activity to enhance the production of interleukin-1 in vivo in a mammalian, and an activity to activate a macrophage in vivo in a mammalian. MI43-37F11 substance may be produced by cultivation of Streptoverticillium eurocidicum MI43-37F11 strain identified as FERM BP-2783.Type: GrantFiled: May 31, 1990Date of Patent: March 17, 1992Assignee: 501 Azidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Masaaki Ishizuka, Hiroyuki Kumagai, Tsutomu Sawa, Hiroshi Naganawa, Hironobu Iinuma, Kunio Isshiki, Masa Hamada, Kenji Maeda, Tomio Takeuchi
-
Patent number: 4686308Abstract: Disclosed is a novel physiologically active substance, MH435, represented by the formula: ##STR1## wherein R represents either of the following groups A and B A: --CH.dbd.CH--NHCHOB: --CH.sub.2 --CH.sub.2 --NHCHO.The substance MH435 has an inhibitory activity against tyrosine specific protein kinase, and the 50% inhibitory concentrations of the substances MH435-A and MH435-B are respectively 0.55 .mu.g/ml and 6.0 .mu.g/ml.Type: GrantFiled: June 26, 1986Date of Patent: August 11, 1987Assignee: Microbial Chemistry Research FoundationInventors: Hamao Umezawa, Tomio Takeuchi, Masa Hamada, Hiroshi Naganawa, Tsutomu Sawa, Masaya Imoto, Hironobu Iinuma, Takeshi Uchida, Kunio Isshiki