Patents by Inventor Kunio Shiota
Kunio Shiota has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9968624Abstract: The invention provides an agent for treating depression, which contains N-acetyl-D-mannosamine, and a pharmaceutical composition for treating depression, which contains an effective amount of N-acetyl-D-mannosamine and a carrier acceptable as a medicament.Type: GrantFiled: May 15, 2015Date of Patent: May 15, 2018Assignee: THE UNIVERSITY OF TOKYOInventors: Kunio Shiota, Shintaro Yagi, Koji Hayakawa, Mitsuko Takamori, Takefumi Kikusui, Yukishige Ito, Yasuharu Sakamoto, Hideharu Seto
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Publication number: 20180085385Abstract: The invention provides an agent containing N-acetyl-D-mannosamine for protecting a glucose-sensitive cell from glucose toxicity due to high glucose concentration; a pharmaceutical composition for protecting a glucose-sensitive cell from glucose toxicity due to high glucose concentration, containing an effective amount of N-acetyl-D-mannosamine and a pharmaceutically acceptable carrier; use of N-acetyl-D-mannosamine in producing a medicament for protecting a glucose-sensitive cell from glucose toxicity due to high glucose concentration; and a method of protecting a glucose-sensitive cell from glucose toxicity due to high glucose concentration, including administering an effective amount of N-acetyl-D-mannosamine to a subject in need thereof. The glucose-sensitive cell is selected from the group consisting of a nerve cell, a renal cell and a vascular endothelial cell.Type: ApplicationFiled: April 1, 2016Publication date: March 29, 2018Applicant: THE UNIVERSITY OF TOKYOInventors: Kunio SHIOTA, Koji HAYAKAWA, Mitsuko TAKAMORI, Ryosuke FUJII, Yukishige ITO
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Publication number: 20170080004Abstract: The invention provides an agent for treating depression, which contains N-acetyl-D-mannosamine, and a pharmaceutical composition for treating depression, which contains an effective amount of N-acetyl-D-mannosamine and a carrier acceptable as a medicament.Type: ApplicationFiled: May 15, 2015Publication date: March 23, 2017Applicant: THE UNIVERSITY OF TOKYOInventors: Kunio SHIOTA, Shintaro YAGI, Koji HAYAKAWA, Mitsuko TAKAMORI, Takefumi KIKUSUI, Yukishige ITO, Yasuharu SAKAMOTO, Hideharu SETO
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Patent number: 9480695Abstract: The invention provides a method for producing an orexin neuron by culturing a pluripotent stem cell or a neural progenitor cell in the presence of N-acetyl-D-mannosamine and optionally in the presence of at least one inhibitor selected from the group consisting of a Sirtuin 1 inhibitor and an O-linked ?-N-acetylglucosamine transferase inhibitor. The invention also provides a therapeutic agent for narcolepsy or eating disorders, such as anorexia, containing N-acetyl-D-mannosamine, which is based on the induction of orexin neuron in vivo.Type: GrantFiled: March 25, 2014Date of Patent: November 1, 2016Assignee: The University of TokyoInventors: Kunio Shiota, Shintaro Yagi, Koji Hayakawa, Mitsuko Hirosawa-Takamori, Daisuke Arai, Keiji Hirabayashi
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Patent number: 9271995Abstract: Provided are effective and highly safe agents, medicaments and the like for ameliorating various disorders caused by brain hypofunction. Also provided is a preventing or ameliorating agent for brain hypofunction containing N-acetyl-D-mannosamine, a pharmaceutical composition for preventing, ameliorating or treating disorders due to brain hypofunction, containing an effective amount of N-acetyl-D-mannosamine and a pharmaceutically acceptable carrier, and a food comprising N-acetyl-D-mannosamine added thereto.Type: GrantFiled: March 18, 2015Date of Patent: March 1, 2016Assignees: The University of Tokyo, School Corporation, Azabu Veterinary Medicine Educational InstitutionInventors: Kunio Shiota, Masayoshi Kuwahara, Takefumi Kikusui, Shintaro Yagi
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Publication number: 20150190414Abstract: Provided are effective and highly safe agents, medicaments and the like for ameliorating various disorders caused by brain hypofunction. Also provided is a preventing or ameliorating agent for brain hypofunction containing N-acetyl-D-mannosamine, a pharmaceutical composition for preventing, ameliorating or treating disorders due to brain hypofunction, containing an effective amount of N-acetyl-D-mannosamine and a pharmaceutically acceptable carrier, and a food comprising N-acetyl-D-mannosamine added thereto.Type: ApplicationFiled: March 18, 2015Publication date: July 9, 2015Inventors: Kunio SHIOTA, Masayoshi KUWAHARA, Takefumi KIKUSUI, Shintaro YAGI
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Patent number: 8987232Abstract: Provided are effective and highly safe agents, medicaments and the like for ameliorating various disorders caused by brain hypofunction. Also provided is a preventing or ameliorating agent for brain hypofunction containing N-acetyl-D-mannosamine, a pharmaceutical composition for preventing, ameliorating or treating disorders due to brain hypofunction, containing an effective amount of N-acetyl-D-mannosamine and a pharmaceutically acceptable carrier, and a food comprising N-acetyl-D-mannosamine added thereto.Type: GrantFiled: February 25, 2011Date of Patent: March 24, 2015Assignees: The University of Tokyo, School Corporation, Azabu Veterinary Medicine Educational InstitutionInventors: Kunio Shiota, Masayoshi Kuwahara, Takefumi Kikusui, Shintaro Yagi
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Publication number: 20140349964Abstract: The invention provides a method for producing an orexin neuron by culturing a pluripotent stem cell or a neural progenitor cell in the presence of N-acetyl-D-mannosamine and optionally in the presence of at least one inhibitor selected from the group consisting of a Sirtuin 1 inhibitor and an O-linked ?-N-acetylglucosamine transferase inhibitor. The invention also provides a therapeutic agent for narcolepsy or eating disorders, such as anorexia, containing N-acetyl-D-mannosamine, which is based on the induction of orexin neuron in vivo.Type: ApplicationFiled: March 25, 2014Publication date: November 27, 2014Inventors: Kunio SHIOTA, Shintaro YAGI, Koji HAYAKAWA, Mitsuko HIROSAWA-TAKAMORI, Daisuke ARAI, Keiji HIRABAYASHI
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Publication number: 20110212917Abstract: Provided are effective and highly safe agents, medicaments and the like for ameliorating various disorders caused by brain hypofunction. Also provided is a preventing or ameliorating agent for brain hypofunction containing N-acetyl-D-mannosamine, a pharmaceutical composition for preventing, ameliorating or treating disorders due to brain hypofunction, containing an effective amount of N-acetyl-D-mannosamine and a pharmaceutically acceptable carrier, and a food comprising N-acetyl-D-mannosamine added thereto.Type: ApplicationFiled: February 25, 2011Publication date: September 1, 2011Applicants: The University of Tokyo, School Corporation, Azabu Veterinary Medicine Educational InstitutionInventors: Kunio SHIOTA, Masayoshi KUWAHARA, Takefumi KIKUSUI, Shintaro YAGI
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Publication number: 20100120036Abstract: To measure the methylation degree of a genomic DNA simply and correctly as well as exhaustively, there is provided a method for amplifying a DNA fragment, characterized by comprising the following steps (1) to (5): (1) digesting a sample double-stranded DNA with a first restriction enzyme; (2) adding a double-stranded DNA fragment consisting of a first oligonucleotide and a second oligonucleotide to a restriction enzyme cleavage end of the double-stranded DNA fragment produced in step (1) to obtain a DNA fragment; (3) digesting the DNA fragment obtained in step (2) with a second restriction enzyme; (4) allowing the double-stranded DNA fragment produced in step (3) to coexist with a third oligonucleotide and a fourth oligonucleotide in the presence of a ligase; and (5) heating the double-stranded DNA fragment obtained in step (4), thereby dissociating the double-stranded DNA fragment and then performing a polymerase chain reaction to selectively amplify a DNA fragment having the cleavage end of the first restType: ApplicationFiled: March 5, 2008Publication date: May 13, 2010Inventors: Kunio Shiota, Shintaro Yagi, Yoko Takahashi, Satoshi Tanaka, Jun Ohgane
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Publication number: 20090221066Abstract: The present invention provides a method of preparing an anti-methylated DNA antibody, comprising a step of administering to an animal an oligodeoxyribonucleotide containing a methylated cytosine(s) and an oligodeoxyribonucleotide containing no methylated cytosine. The antibody prepared by this method is capable of detecting a DNA containing a methylated cytosine(s) in a state close to the state of a biological component.Type: ApplicationFiled: March 27, 2007Publication date: September 3, 2009Inventors: Kunio Shiota, Shintaro Yagi, Fumiko Sunaga, Keiji Hirabayashi
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Publication number: 20060134659Abstract: It is an object of the present invention to provide a demethylation method that, from among multiple methylated regions present in the genome DNA of a living cell, can demethylate a specified methylated region only without influencing the other methylated regions, and to achieve the object, a target methylated region present in either a sense or antisense DNA chain in genome DNA of a living cell is demethylated, by artificially generating in the living cell the following (a) RNA comprising a base sequence equivalent to the base sequence of a region comprising all or part of the target methylated region in the DNA chain where the target methylated region is present, or (b) RNA comprising a base sequence equivalent to the base sequence of a region comprising a region neighboring the target methylated region in the DNA chain where the target methylated region is present.Type: ApplicationFiled: September 6, 2005Publication date: June 22, 2006Applicant: TOUDAI TLO, Ltd.Inventors: Kunio Shiota, Satoshi Tanaka, Jun Ohgane, Takuya Imamura, Naka Hattori, Koichiro Nishino, Soshi Yamamoto, Naoko Hattori
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Publication number: 20020072059Abstract: The present invention provides a method of identifying a cell, tissue or nucleus, comprising collecting information on the methylation pattern of DNA isolated from the cell, tissue or nucleus and analyzing the resultant information.Type: ApplicationFiled: June 18, 2001Publication date: June 13, 2002Applicant: The University of TokyoInventors: Kunio Shiota, Satoshi Tanaka, Jun Ohgane, Naka Hattori