Abstract: Provided is a method of reconstructing multi-source long-time-series night light data and a system thereof, relating to the field of reconstructing ecological remote sensing data. Based on night light images of a first-generation satellite DMSP/OLS (Defense Meteorological Satellite Program/Operational Line-scan System) and a second-generation satellite NPP/VIIRS (National Polar-orbiting Partnership/Visible Infrared Imaging Radiometer Suite), a method of reconstructing a set of long-time-series night light data products is developed. Since satellite images that two generations of satellites have at the same time in a certain year use an inverse hyperbolic sine transform to fit NPP/VIIRS in 2013 into a data form of DMSP/OLS, and obtain an optimal fitting equation, thus producing a set of long-time-series data products from 1992 to 2021. The method can solve a fault problem between two generations of light data of DMSP/OLS and NPP/VIRS, and improve the accuracy of data reconstruction.

Abstract: The present invention relates to a recombinant HBV cccDNA comprising HBV genome or the fragment or variant thereof and a site-hybrid insert, a method to generate said recombinant HBV cccDNA, a method for establishment of an in vitro or in vivo cccDNA based model for persistently hepatitis B virus replication by using the recombinant HBV cccDNA of the present invention, and a method for anti-HBV drug evaluation.