Abstract: A cell culture device comprises a top layer, a diffusion layer, and a suspension layer. The diffusion layer comprises at least one diffusion channel. The suspension layer comprises multiple suspension units. The multiple suspension units are connected to the diffusion channel. A fluid is conducted through the diffusion channel and flow into the suspension units for drop suspended. Cells are cultivated in the fluid suspended in the suspension unit. The cell culture device can be used in an electromagnetic cell culturing system. The electromagnetic cell culturing system is a combination of hanging drop method, microfluidic technology and electromagnetic stimulation. The electromagnetic cell culturing system can improve the growth of cells and also is capable of continuously providing culture medium into the cell culture device. The cell culture device can be used to cultivate large quantity of cells with high productivity and stable quality.