Abstract: The present disclosure relates to methods of differentiating pluripotent stem cells into endothelial cells. The present disclosure also relates to endothelial cells made by such methods, organoids containing such endothelial cells, and methods of use thereof. The present disclosure also relates to differentiation media for use in the same.

Abstract: A wellbore material transfer system is disclosed, and comprises a downhole fluid conductor defining a downhole-conducting fluid passage, an uphole fluid conductor defining an uphole-conducting fluid passage, a flow controller for opening and closing the uphole-conducting fluid passage, and a completion disposed within a wellbore and defining a wellbore interval between the completion and the wellbore, the completion including selectively openable first and second flow communicators. While the flow communicators are open and the uphole-conducting fluid passage is closed, a subterranean formation zone may be fractured, and flow communication is absent, via the second flow communicator and the uphole-conducting fluid passage, between the wellbore interval and the surface.

Abstract: There is provided a process for stimulating hydrocarbon production from a subterranean formation, comprising: conducting stimulation material to the subterranean formation via a wellbore, wherein the stimulation material includes solid particulate material; within the wellbore, agitating the conducted stimulation material with a fluid motion-actuating tool, such as, for example, a coiled tubing agitator tool.

Abstract: There is provided a process for stimulating hydrocarbon production from a subterranean formation, comprising: conducting stimulation material to the subterranean formation via a wellbore, wherein the stimulation material includes solid particulate material; within the wellbore, agitating the conducted stimulation material with a fluid motion-actuating tool, such as, for example, a coiled tubing agitator tool.