Patents by Inventor Kurt Greenizen

Kurt Greenizen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11925904
    Abstract: A funnel-less filtration device that attaches directly to a storage container such as a cell culture media bottle. The device includes a filter collar containing one or more membranes, an inlet with a coupling device for attaching the filter device to a supply of liquid to be filtered, an outlet at a lower portion of the collar, a vacuum port in the collar below the membrane(s) and a filtered vent in the collar above the membrane(s). Optionally, the device may include a filtrate reservoir attached to the outlet, preferably by a threaded connection. Optionally, the upper opening to the filter collar is selectively and removably sealed with a lid until used. The lid is then removed the filter device inverted over a storage container such as cell culture media bottle and the two are attached via the upper opening of the filter collar. The assembly is then inverted so the supply container is above the filter device.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: March 12, 2024
    Assignee: EMD Millipore Corporation
    Inventors: Christopher A. Scott, Kurt Greenizen, Paul Sydlowski
  • Publication number: 20210299614
    Abstract: A funnel-less filtration device that attaches directly to a storage container such as a cell culture media bottle. The device includes a filter collar containing one or more membranes, an inlet with a coupling device for attaching the filter device to a supply of liquid to be filtered, an outlet at a lower portion of the collar, a vacuum port in the collar below the membrane(s) and a filtered vent in the collar above the membrane(s). Optionally, the device may include a filtrate reservoir attached to the outlet, preferably by a threaded connection. Optionally, the upper opening to the filter collar is selectively and removably sealed with a lid until used. The lid is then removed the filter device inverted over a storage container such as cell culture media bottle and the two are attached via the upper opening of the filter collar. The assembly is then inverted so the supply container is above the filter device.
    Type: Application
    Filed: October 5, 2017
    Publication date: September 30, 2021
    Inventors: Christopher A. Scott, Kurt Greenizen, Paul Sydlowski
  • Patent number: 11104491
    Abstract: A container and a container engaging member. The container engaging member may include a sample holder or reservoir, a filtration element and collar. In the assembled condition, the sample holder or reservoir is upstream of the filtration element, the container is downstream of the filtration element, and the sample holder or reservoir is attached to the container. The container engagement member is engageable and disengageable from the bottle or container in a quick attach, quick release manner, such as with only a 90 degree, ¼ turn. A tactile and/or audible indication that the engagement is complete is provided.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: August 31, 2021
    Assignee: EMD Millipore Corporation
    Inventors: Chris Scott, Kurt Greenizen, Paul Sydlowski, Jay Doyle
  • Publication number: 20200360918
    Abstract: An apparatus for simultaneously extracting one or more analytes from a plurality of samples, the apparatus comprising a housing having an upper platform having a plurality of pins, each pin suitable for BioSPME, and a lower platform having a plurality of wells for sample solutions, such when the upper platform is fitted over the lower platform, the pins of the upper platform fit into the wells of the lower platform such that the pins are immersed into the samples, allowing for simultaneous extraction of analytes in each sample. The apparatus can be coupled with other instruments to enable measurement of total and free analytes in each sample. Also provided are methods of simultaneous extraction of analytes in a plurality of samples using the apparatus described herein.
    Type: Application
    Filed: August 14, 2018
    Publication date: November 19, 2020
    Inventors: Yong Chen, Olga I. Shimelis, Dave Bell, Katherine K. Stenerson, Craig Aurund, Emily Barrey, Xiaoning Lu, Kurt Greenizen
  • Patent number: 10656060
    Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
    Type: Grant
    Filed: August 29, 2018
    Date of Patent: May 19, 2020
    Assignee: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, John Doyle
  • Publication number: 20180372600
    Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
    Type: Application
    Filed: August 29, 2018
    Publication date: December 27, 2018
    Inventors: Phillip Clark, Kurt Greenizen, John Doyle
  • Patent number: 10088398
    Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: October 2, 2018
    Assignee: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, John Doyle
  • Publication number: 20180079560
    Abstract: A container and a container engaging member. The container engaging member may include a sample holder or reservoir, a filtration element and collar. In the assembled condition, the sample holder or reservoir is upstream of the filtration element, the container is downstream of the filtration element, and the sample holder or reservoir is attached to the container. The container engagement member is engageable and disengageable from the bottle or container in a quick attach, quick release manner, such as with only a 90 degree, ¼ turn. A tactile and/or audible indication that the engagement is complete is provided.
    Type: Application
    Filed: November 29, 2017
    Publication date: March 22, 2018
    Inventors: Chris Scott, Kurt Greenizen, Paul Sydlowski, Jay Doyle
  • Patent number: 9897520
    Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: February 20, 2018
    Assignee: EMD Millipore Corporation
    Inventors: Christopher A. Scott, Timothy K. Nadler, Kurt Greenizen, Louis Bonhomme, Sara D. Gutierrez, Masaharu Mabuchi, David Briggs, Phillip Clark, Ralph T. Scaduto
  • Patent number: 9850040
    Abstract: A container and a container engaging member. The container engaging member may include a sample holder or reservoir, a filtration element and collar. In the assembled condition, the sample holder or reservoir is upstream of the filtration element, the container is downstream of the filtration element, and the sample holder or reservoir is attached to the container. The container engagement member is engageable and disengageable from the bottle or container in a quick attach, quick release manner, such as with only a 90 degree, ¼ turn. A tactile and/or audible indication that the engagement is complete is provided.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: December 26, 2017
    Assignee: EMD Millipore Corporation
    Inventors: Chris Scott, Kurt Greenizen, Paul Sydlowski, Jay Doyle
  • Patent number: 9783769
    Abstract: The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall.
    Type: Grant
    Filed: July 16, 2009
    Date of Patent: October 10, 2017
    Assignee: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, Christopher A. Scott, Marc Emerick
  • Publication number: 20160228870
    Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
    Type: Application
    Filed: January 13, 2016
    Publication date: August 11, 2016
    Inventors: Phillip Clark, Kurt Greenizen, John Doyle
  • Publication number: 20160137961
    Abstract: The present invention is a cell cultivating vessel or device, such as a single or multitier flask, including a cover having a top plate, a side wall and a resealable port; an intermediate tray for receiving cells and cell culture media, having a bottom plate, a side wall, and a gap region formed between an interior upwardly angled lip located on an interior portion of the intermediate tray bottom plate and an adjacent outwardly angled side wall portion of the intermediate tray bottom plate, wherein the lip has a outwardly swooping curvilinear edge feature; and a base tray for receiving the cells and cell culture media, including a bottom plate and a side wall.
    Type: Application
    Filed: July 16, 2009
    Publication date: May 19, 2016
    Applicant: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, Christopher A. Scott, Marc Emerick
  • Publication number: 20160103045
    Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
    Type: Application
    Filed: December 18, 2015
    Publication date: April 14, 2016
    Inventors: Christopher A. Scott, Timothy K. Nadler, Kurt Greenizen, Louis Bonhomme, Sara D. Gutierrez, Masaharu Mabuchi, David Briggs, Phillip Clark, Ralph T. Scaduto
  • Patent number: 9304070
    Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
    Type: Grant
    Filed: June 27, 2012
    Date of Patent: April 5, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Christopher A. Scott, Timothy K. Nadler, Kurt Greenizen, Louis Bonhomme, Sara D. Gutierrez, Masaharu Mabuchi, David Briggs, Phillip Clark, Ralph T. Scaduto
  • Patent number: 9295947
    Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: March 29, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Louis Bonhomme, John Doyle, Kurt Greenizen, George Gagne, Sean Devin, Gerard Lombard, Vincent Smith
  • Publication number: 20150266630
    Abstract: A container and a container engaging member. The container engaging member may include a sample holder or reservoir, a filtration element and collar. In the assembled condition, the sample holder or reservoir is upstream of the filtration element, the container is downstream of the filtration element, and the sample holder or reservoir is attached to the container. The container engagement member is engageable and disengageable from the bottle or container in a quick attach, quick release manner, such as with only a 90 degree, ¼ turn. A tactile and/or audible indication that the engagement is complete is provided.
    Type: Application
    Filed: March 9, 2015
    Publication date: September 24, 2015
    Inventors: Chris Scott, Kurt Greenizen, Paul Sydlowski, Jay Doyle
  • Patent number: 9103756
    Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: August 11, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Christopher A. Scott, Timothy K. Nadler, Kurt Greenizen, Louis Bonhomme, Sara D. Gutierrez, Masaharu Mabuchi, David Briggs, Phillip Clark, Ralph T. Scaduto
  • Patent number: 9050565
    Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device.
    Type: Grant
    Filed: January 11, 2013
    Date of Patent: June 9, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Louis Bonhomme, John Doyle, Kurt Greenizen, George Gagne, Sean Devin, Gerard Lombard, Vincent Smith
  • Patent number: D804653
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: December 5, 2017
    Assignee: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, Paul Sydlowski, Christopher A. Scott