Patents by Inventor Kwang Lae Hoe

Kwang Lae Hoe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Screening method for drug target gene using heterozygous deletion fission yeast strain
    Patent number: 10174312
    Abstract: The present invention relates to a screening method for a drug target gene by using chemical-genetic profile compendium of the heterozygous deletion fission yeast strain and the comparative genetic analysis using the same. More precisely, the present inventors constructed the chemical-genetic profile compendium for drug candidates from the heterozygous deletion fission yeast strain of Schizosaccharomyces pombe (S. pombe), and then compared with the compendium with the chemical-genetic profile compendium of the budding yeast Saccharomyces cerevisiae (S. cerevisiae) in order to select efficiently drug target genes showing drug sensitivity. The screening method of the present invention can be efficiently used for the identification of a drug target gene in various eukaryotes because it facilitates the selection of a drug target gene showing sensitivity to the drug from the chemical-genetic profile compendium of the heterozygous deletion fission yeast strain.
    Type: Grant
    Filed: June 2, 2015
    Date of Patent: January 8, 2019
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Dong-Uk Kim, Kwang-Lae Hoe
  • Genome-wide construction of heterozygous deletion mutants containing gene-specific barcodes by the methods of 4-round serial or block PCR, or total gene synthesis thereof
    Patent number: 9670480
    Abstract: A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: June 6, 2017
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Kwang Lae Hoe, Dong Uk Kim, Mi Sun Won, Hyang Sook Yoo, Dong Sup Kim, Han Oh Park, Kyung Sook Chung, Young Joo Jang, Mi Young Nam, Sang Jo Han, Shin Jung Choi, Seung Tae Baek, Hyong Bai Kim, Kyung Sun Heo, Hye Mi Lee, Min Ho Lee, Jo Young Park
  • Screening method for drug target gene using heterozygous deletion fission yeast strain
    Publication number: 20150344874
    Abstract: The present invention relates to a screening method for a drug target gene by using chemical-genetic profile compendium of the heterozygous deletion fission yeast strain and the comparative genetic analysis using the same. More precisely, the present inventors constructed the chemical-genetic profile compendium for drug candidates from the heterozygous deletion fission yeast strain of Schizosaccharomyces pombe (S. pombe), and then compared with the compendium with the chemical-genetic profile compendium of the budding yeast Saccharomyces cerevisiae (S. cerevisiae) in order to select efficiently drug target genes showing drug sensitivity. The screening method of the present invention can be efficiently used for the identification of a drug target gene in various eukaryotes because it facilitates the selection of a drug target gene showing sensitivity to the drug from the chemical-genetic profile compendium of the heterozygous deletion fission yeast strain.
    Type: Application
    Filed: June 2, 2015
    Publication date: December 3, 2015
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Dong-Uk KIM, Kwang-Lae HOE
  • Genome-Wide Construction of Schizosaccharomyces Pombe Heterozygous Deletion Mutants Containing Gene-Specific Barcodes by the Methods of 4-Round Serial or Block PCR, or Total Gene Synthesis Thereof
    Publication number: 20110190163
    Abstract: A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
    Type: Application
    Filed: August 27, 2008
    Publication date: August 4, 2011
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Kwang Lae Hoe, Dong Uk Kim, Mi Sun Won, Hyang Sook Yoo, Dong Sup Kim, Han Oh Park, Kyung Sook Chung, Young Joo Jang, Mi Young Nam, Sang Jo Han, Hin Jung Choi, Seung Tae Baek, Hyong Bai Kim, Kyung Sun Heo, Hye Mi Lee, Min Ho Lee, Jo Young Park