Abstract: An electric motor includes a stator and a rotor rotatable relative to the stator. The rotor includes an axial shaft, a disc projecting radially from the shaft, and a ring magnet affixed to the disc. The shaft and the disc are integrally formed as a unitary body.

Abstract: The present invention provides methods for preparing nucleic acid samples. The methods of the present invention are particularly amenable for preparing samples that substantially represent the whole transcripts. In some aspects the methods include a step of reducing the amount of ribosomal RNA in a total RNA sample prior to amplification. In preferred aspects single stranded sense strand cDNA is generated, labeled and hybridized to arrays of probes. The method is particularly suitable to use with microarray based expression analysis.

Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.

Abstract: The present invention provides methods for preparing nucleic acid samples. The methods of the present invention are particularly amenable for preparing samples that substantially represent the whole transcripts. The method is particularly suitable to use with microarray based expression analysis.

Abstract: In one aspect of the invention, methods are provided for the creation and screening of polypeptides that eliminates bacterial cloning and individual screening In preferred embodiments, the method involves partnering each protein with a unique DNA oligonucleotide tag that directs the protein to a unique site on the microarray due to specific hybridization with a complementary tag-probe on the array

Abstract: Methods and kits for amplifying nucleic acids are provided. Double stranded cDNA with uridine residues incorporated into one strand is synthesized and the uridine containing strand is nicked at uridine residues. The DNA is extended from the nicks in the presence of dUTP using a strand displacing enzyme. Repeated cycles of nicking and extension result in amplification of the nucleic acid. Methods are also provided for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the collection of target sequences for particular characteristics, such as, for example, the presence or absence of one or more polymorphisms or the presence or absence of a transcript.

Abstract: In one aspect of the invention, methods and compositions are provided for fragmenting nucleic acid samples. Fragmented nucleic acid samples may be used for hybridization with microarrays.

Abstract: Methods are provided for enhancing generation of cDNA strands from mRNA. These methods are particularly useful for generating sufficient quantities of target for microarray hybridization.

Abstract: In one aspect of the present invention, a method for preparing a nucleic acid sample for hybridization to a nucleic acid array is presented. This disclosed method has the steps of providing a nucleic acid sample, the sample having mRNA, amplifying the mRNA to produce cRNA and fragmenting the cRNA with an RNase enzyme to produce fragments. In another aspect of the present invention, a method is provided for detecting hybridization of a nucleic acid sample to a nucleic acid array. In yet another aspect of the present invention, a method is presented for preparing cRNA for hybridization to an oligonucleotide probe array. In another aspect of the present invention, a method is provided for labeling an RNA sample, the method comprising providing an RNA sample, fragmenting the sample with an RNase enzyme to produce fragments and end-labeling the fragments with a detectable label.