Abstract: An example welding system includes a power supply configured to output welding power; a weld parameter feedback sensor configured to produce a weld feedback parameter corresponding to an actual weld condition during welding; and a welding program comprising: a first weld having first weld parameters; a second weld having second weld parameters, wherein the second weld follows the first weld; a controller configured to, in response to selection of the weld program for performing a welding job on a part: control the power supply to provide the welding power to a first weld operation based the first weld parameters; monitor first feedback from the power supply; control the power supply to provide the welding power to a second weld operation based the second weld parameters; monitor second feedback from the power supply; and determine whether at least one of the welding job or the part is acceptable.

Abstract: Disclosed herein are systems, compositions and methods for using a split dCas protein system to modify the epigenetic profile of a gene of interest. The systems, compositions, and methods are useful for modifying the epigenetic profile of a particular gene within a cell, based on the discovery that effective expression of a larger-sized recombinant protein can be successfully achieved using two separate expression cassettes each encoding a half of the protein fused with a half of an intein, utilizing the unique feature of an intein system to ultimately rejoin the two halves to form one larger fusion protein with the intein spliced out.

Abstract: A gene editing system is provided that comprises a first nucleotide molecule encoding a dCas9-Ten-Eleven Translocation methylcytosine dioxygenase 1 catalytic domain (TET1CD) fusion protein; and a second nucleotide molecule encoding at least one small guide RNA (sgRNA), comprising: a scaffold region and a spacer region, wherein the spacer region hybridizes to a nucleotide sequence complementary to a target sequence adjacent to a 5?-end of a protospacer adjacent motif (PAM), and wherein the target sequence and the PAM are located within 1 kilobase (kb) of the transcriptional start site (TSS) of the CDKL5 gene. Methods of making and using the system are further described herein.

Abstract: An example welding system includes a power supply configured to output welding power; a weld parameter feedback sensor configured to produce a weld feedback parameter corresponding to an actual weld condition during welding; and a welding program comprising: a first weld having first weld parameters; a second weld having second weld parameters, wherein the second weld follows the first weld; a controller configured to, in response to selection of the weld program for performing a welding job on a part: control the power supply to provide the welding power to a first weld operation based the first weld parameters; monitor first feedback from the power supply; control the power supply to provide the welding power to a second weld operation based the second weld parameters; monitor second feedback from the power supply; and determine whether at least one of the welding job or the part is acceptable.

Abstract: A welding system including an arc monitoring, training, and control system is disclosed. The welding system includes a power supply, controller, and associated memory. When a weld is performed, the weld command and weld feedback parameters can be stored in the memory, along with associated alarm limit values. During subsequent welds, the input weld commands and actual feedback values can be compared to the established limits, and a fault signal provided to an operator or supervisor when the value exceeds the established limits. The fault signals can be used for training operators, as well as providing monitoring signals, and can be stored with weld data in a database for later analysis. In addition, collected weld data can be used to determine when to clean, repair, or replace consumables, including, for example, contact tips, wire drive liners, and drive rolls, and to monitor usage of wire and gas.

Abstract: A welding system including an arc monitoring, training, and control system is disclosed. The welding system includes a power supply, controller, and associated memory. When a weld is performed, the weld command and weld feedback parameters can be stored in the memory, along with associated alarm limit values. During subsequent welds, the input weld commands and actual feedback values can be compared to the established limits, and a fault signal provided to an operator or supervisor when the value exceeds the established limits. The fault signals can be used for training operators, as well as providing monitoring signals, and can be stored with weld data in a database for later analysis. In addition, collected weld data can be used to determine when to clean, repair, or replace consumables, including, for example, contact tips, wire drive liners, and drive rolls, and to monitor usage of wire and gas.

Abstract: Embodiments of a welding system include a compact wire feeder adapted to feed welding wire having a diameter up to approximately 5/64 inches disposed on a wire spool having an outer diameter of approximately 8 inches. The compact wire feeder may include a housing, a spool mounting hub disposed in the housing and adapted to receive the wire spool, and a wire drive assembly having a motor adapted to apply an output torque to a shaft of a gearbox to drive the welding wire from the welding spool to a welding torch.