Patents by Inventor Kyle Kinealy

Kyle Kinealy has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography
    Patent number: 8969532
    Abstract: The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.
    Type: Grant
    Filed: October 3, 2007
    Date of Patent: March 3, 2015
    Assignee: Novo Nordisk A/S
    Inventors: Shawn DeFrees, Kyle Kinealy
  • Tagged sialyltransferase proteins
    Patent number: 7968310
    Abstract: The present invention provides modified Campylobacter sialyltransferase proteins that exhibit enhanced expression as compared to its unmodified form. Nucleic acids that encode the sialyltransferase proteins are also included, as are methods to produce and use the sialyltransferase proteins.
    Type: Grant
    Filed: February 1, 2007
    Date of Patent: June 28, 2011
    Assignee: BioGeneriX AG
    Inventors: Karl Johnson, Aliakbar Mobasseri, Xiaomei Bai, Kyle Kinealy
  • METHODS FOR THE PURIFICATION OF POLYPEPTIDE CONJUGATES
    Publication number: 20100075375
    Abstract: The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.
    Type: Application
    Filed: October 3, 2007
    Publication date: March 25, 2010
    Inventors: Shawn Defrees, Kyle Kinealy
  • METHODS FOR THE PURIFICATION OF POLYPEPTIDE CONJUGATES
    Publication number: 20100035299
    Abstract: The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.
    Type: Application
    Filed: April 3, 2009
    Publication date: February 11, 2010
    Applicant: Novo Nordisk A/S
    Inventors: Shawn DeFrees, Kyle Kinealy
  • MODIFICATIONS OF CST-II FOR INCREASED PROTEIN EXPRESSION
    Publication number: 20090186377
    Abstract: The present invention provides modified Campylobacter sialyltransferase proteins that exhibit enhanced expression as compared to its unmodified form. Nucleic acids that encode the sialyltransferase proteins are also included, as are methods to produce and use the sialyltransferase proteins.
    Type: Application
    Filed: February 1, 2007
    Publication date: July 23, 2009
    Inventors: Karl Johnson, Aliakbar Mobaserri, Xiaomei Bai, Kyle Kinealy
  • METHODS FOR THE PURIFICATION OF POLYPEPTIDE CONJUGATES
    Publication number: 20080253992
    Abstract: The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.
    Type: Application
    Filed: October 3, 2007
    Publication date: October 16, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, Kyle Kinealy
  • MANUFACTURING PROCESS FOR THE PRODUCTION OF POLYPEPTIDES EXPRESSED IN INSECT CELL-LINES
    Publication number: 20080207487
    Abstract: The present invention provides a manufacturing method for polypeptides that are produced in insect cells using a baculoviral expression system. In one example, the insect cell culture is supplemented with a lipid mixture immediately prior to infection (e.g., one hour prior to infection). The polypeptides are isolated from the insect cell culture using a method that employs anion exchange or mixed-mode chromatography early in the purification process. This process step is useful to remove insect-cell derived endoglycanases and proteases and thus reduces the loss of desired polypeptide due to enzymatic degradation. In another example, mixed-mode chromatography is combined with dye-ligand affinity chromatography in a continuous-flow manner to allow for rapid processing of the insect-cell culture liquid and capture of the polypeptide.
    Type: Application
    Filed: November 2, 2007
    Publication date: August 28, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, Kyle Kinealy, Sibylle Herzer