Abstract: Therapeutic chondrisome compositions and related methods are described.

Abstract: Therapeutic chondrisome and mitoplast compositions and related methods are described.

Abstract: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can fuse to the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Abstract: Fusosome compositions and methods are described herein.

Abstract: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Abstract: Provided herein are methods of transducing resting or non-activated T cells using CD8-targeted viral vectors.

Abstract: Therapeutic chondrisome compositions and related methods are described.

Abstract: Provided herein are lipid particles containing a lipid bilayer enclosing a lumen or cavity, a henipavirus F protein molecule or biologically active portion thereof, and a targeted envelope protein containing a henipavirus envelope attachment glycoprotein G (G protein) or biologically active portion thereof and a binding domain, such as a single domain antibody (sdAb) variable domain. Also provided herein are targeted envelope proteins containing a G protein fused or linked to a binding domain, such as a sdAb variable domain, and polynucleotides encoding such proteins. Also provided are producer cells and compositions containing such targeted lipid particles and methods of making and using the targeted lipid particles.

Abstract: The present disclosure provides, at least in part, methods and compositions for in vivo fusosome delivery. In some embodiments, the fusosome comprises a combination of elements that promote specificity for target cells, e.g., one or more of a re-targeted fusogen, a positive target cell-specific regulatory element, and a non-target cell-specific regulatory element. In some embodiments, the fusosome comprises one or more modifications that decrease an immune response against the fusosome.

Abstract: This disclosure provides, e.g., cytobiologic compositions and methods of use thereof. The cytobiologics can be used, e.g., to deliver a protein or nucleic acid to a target cell.

Abstract: Fusosome compositions and methods are described herein.

Abstract: In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Abstract: Therapeutic chondrisome compositions and related methods are described.

Abstract: A system to provide control architecture comprises a control, a client and a client container. The control can hold client-side resources for an appropriate resource scope to achieve optimal performance and utilization of resources, wherein access to the client-side resources is parameterized by metadata annotation declared on the control. The client is capable of at least one of the following via the control performing a plurality of operations to access and/or configure the plurality of properties of the resource; and retrieving data from the resource. In addition, the client container is capable of maintaining an accumulated list of controls that have acquired resources; and releasing resources at the end of appropriate resource scope. The definition of the resource scope can be delegated to the client container within which the control is executing.

Abstract: A developer can express the logic offered by a Web service, or other network accessible service, using a standard programming language augmented with declarative annotations specifying preferences for exposing that logic as a Web service. At compile time, an enhanced compiler can analyze the annotated source file and automatically generate the mechanisms required to expose its functionality as a Web service or network accessible service.

Abstract: The deployment and management of differing versions of software components can be simplified. External interfaces of the component can remain constant in behavior while the internal logic, and possibly internal storage, can change between versions. Such a version management system can be transparent to the client programs, since interfaces can remain backwards compatible between component versions. This transparency can greatly reduce the risks of unpredictable behavior or system failure. A system can maintain each version of a software component, and can use a dispatch technique to determine and instantiate the currently active version whenever a client requests a service from the software component during a session. Old instances, possibly of other versions, can be maintained as long as required on the server. Such systems can apply to stateful and stateless components, using synchronous or asynchronous communications, and which may communicate over networks and may use web services type protocols.

Abstract: An extensible container framework can be used to create and deploy software runtime containers. Such a framework can allow containers to inherit functionality and runtime environment attributes of other such containers. An extensible container framework can support multiple component types, and can provide the ability to add incremental features through a common component architecture. A framework can provide a common set of container infrastructure and services, and can expand the leverage of capabilities built around each container. This description is not intended to be a complete description of, or limit the scope of, the invention. Other features, aspects, and objects of the invention can be obtained from a review of the specification, the figures, and the claims.

Abstract: A system to provide control architecture comprises a control, a client and a client container. The control can hold client-side resources for an appropriate resource scope to achieve optimal performance and utilization of resources, wherein access to the client-side resources is parameterized by metadata annotation declared on the control. The client is capable of at least one of the following via the control performing a plurality of operations to access and/or configure the plurality of properties of the resource; and retrieving data from the resource. In addition, the client container is capable of maintaining an accumulated list of controls that have acquired resources; and releasing resources at the end of appropriate resource scope. The definition of the resource scope can be delegated to the client container within which the control is executing.

Abstract: Controls can reduce the complexity and learning curve associated with acting as a resource client by utilizing a unified client model that can provide access to diverse types of resources, the configuration options, operations, and events exposed by a resource. Controls can also provide transparent (to the client) resource management of connections, sessions, or other resources to be obtained on behalf of the client. In addition, an external configuration module can be utilized to define how properties can be bounded by external configurations, enabling the overriding of attributes of the resource at deployment time.

Abstract: A system and method for creating a proxy object capable of communication with an external entity, comprising specifying a proxy object definition for the proxy object wherein the proxy object definition defines a first function for communicating with the external entity, specifying at least one implementation class for the proxy object definition, wherein the at least one implementation class does not implement the first function, and wherein the at least one implementation class includes functionality to support one of: proxy object design, software compilation and software execution.