Patents by Inventor Kyle Richard Gee
Kyle Richard Gee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120040380Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a ?-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a ?-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a ?-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a ?-lactmase or instructions for using the components in an assay.Type: ApplicationFiled: June 22, 2011Publication date: February 16, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: SCHUYLER BOON CORRY, WILLIAM LOUIS DOWNEY, BRIAN FILANOSKI, KYLE RICHARD GEE, I. LAWRENCE GREENFIELD, JAMES DAVID HIRSCH, IAIN JOHNSON, ALEKSEY RUKAVISHNIKOV
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Publication number: 20120009683Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.Type: ApplicationFiled: July 29, 2011Publication date: January 12, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle Richard GEE, Vladimir MARTIN
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Publication number: 20110171736Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.Type: ApplicationFiled: November 11, 2010Publication date: July 14, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Brian AGNEW, Kyle Richard Gee
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Publication number: 20100196922Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.Type: ApplicationFiled: December 31, 2009Publication date: August 5, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle Richard GEE, Vladimir Martin
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Publication number: 20100081203Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.Type: ApplicationFiled: June 19, 2009Publication date: April 1, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Brian Agnew, Kyle Richard Gee
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Publication number: 20090047692Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a ?-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a ?-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a ?-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a ?-lactmase or instructions for using the components in an assay.Type: ApplicationFiled: April 7, 2008Publication date: February 19, 2009Applicant: INVITROGEN CORPORATIONInventors: Schuyler Boon CORRY, William Louis Downey, Brian Filanoski, Kyle Richard Gee, I. Lawrence Greenfield, James David Hirsch, Iain Johnson, Aleksey Rukavishnikov
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Publication number: 20090029389Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.Type: ApplicationFiled: July 25, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventors: Kyle Richard Gee, Vladimir Martin
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Publication number: 20090017546Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.Type: ApplicationFiled: July 21, 2008Publication date: January 15, 2009Applicant: INVITROGEN CORPORATIONInventors: Brian Agnew, Kyle Richard Gee
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Publication number: 20090004641Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.Type: ApplicationFiled: October 11, 2007Publication date: January 1, 2009Applicant: INVITROGEN CORPORATIONInventors: Kyle Richard Gee, Courtenay Rae Hart, Richard Haugland, Wayne Forrest Patton, Scott Whitney
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Patent number: 7282339Abstract: The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample.Type: GrantFiled: September 17, 2004Date of Patent: October 16, 2007Assignee: Invitrogen CorporationInventors: Joseph Beechem, Kyle Richard Gee, David Carl Hagen, Iain D Johnson, Hee-Chol Kang, Christina Pastula