Abstract: Provided is a method of operating an apparatus for prostate cancer analysis operated by at least one processor, the method including: receiving digital slide images prepared from serial sections of a prostatectomy specimen, and a gross image of the serial sections; acquiring prostate cancer-related histological information of each received digital slide image using an artificial neural network model trained to infer histological information from the digital slide images; generating digital pathology images by displaying the prostate cancer-related histological information inferred from the artificial neural network model on each digital slide image; and providing a histological mapping image in which a tumor region extracted from each digital pathology image is mapped to a gross image of the corresponding section.