Patents by Inventor Kyriacos Mitrophanous
Kyriacos Mitrophanous has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230183745Abstract: A cell for producing retroviral vectors comprising nucleic acid sequences encoding: i) gag-pol; ii) env; iii) the RNA genome of the retroviral vector; and iv) optionally rev, or a functional substitute thereof, wherein at least two nucleic acid sequences are located at the same genetic locus; and wherein the at least two nucleic acid sequences are in reverse and/or alternating orientations.Type: ApplicationFiled: February 7, 2023Publication date: June 15, 2023Inventors: Hannah Stewart, Helen Maunder, Kyriacos Mitrophanous, Daniel Farley
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Publication number: 20220175962Abstract: A method of improving motor function and reducing dyskinesia in a subject suffering from a neurodegenerative disease or a disease where endogenous dopamine levels are reduced in the subject comprising administering an effective amount of a viral vector comprising a nucleic acid construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1), (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC), or any combination thereof to the subject.Type: ApplicationFiled: March 10, 2020Publication date: June 9, 2022Inventors: Gavin CORCORAN, Michele STONE, Kyriacos MITROPHANOUS, Hannah STEWART
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Patent number: 11279954Abstract: Provided is a construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1) and (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC) wherein the nucleotide sequence encoding TH is linked to the nucleotide sequence encoding CH1 such that they encode a fusion protein TH-CH1. Also provided is a construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1) and (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC) wherein the nucleotide sequence encoding AADC is linked to the nucleotide sequence encoding TH such that they encode a fusion protein AADC-TH or TH-AADC. Further provided is a viral vector comprising such nucleotide sequences and its use in the treatment and/or prevention of Parkinson's disease.Type: GrantFiled: June 26, 2019Date of Patent: March 22, 2022Assignee: Oxford BioMedica (UK) Ltd.Inventors: Kyriacos A. Mitrophanous, Scott Ralph, Hannah Stewart, Alan John Kingsman
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Publication number: 20210079360Abstract: Disclosed herein are viral vector production systems secreting nuclease for degradation of residual nucleic acid during viral vector production and methods of the same. Such a viral vector production system comprises a viral vector production cell comprising nucleic acid sequences encoding: 1) viral vector components; and 2) a nuclease, wherein the nuclease is expressed in the production cell and secreted in cell culture thereby degrading residual nucleic acid during viral vector production. Another such viral vector production system comprises 1) a viral vector production cell comprising nucleic acid sequences encoding viral vector components; and 2) a nuclease helper cell comprising a nucleic acid sequence encoding a nuclease, wherein the nuclease is expressed and secreted in co-culture of the production cell of 1) and the helper cell of 2), thereby degrading residual nucleic acid during viral vector production.Type: ApplicationFiled: March 15, 2019Publication date: March 18, 2021Inventors: Daniel Farley, Kyriacos Mitrophanous
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Publication number: 20200102578Abstract: The present invention relates to a nucleic acid sequence comprising a binding site operably linked to a nucleotide of interest, wherein the binding site is capable of interacting with an RNA-binding protein such that translation of the nucleotide of interest is repressed in a viral vector production cell.Type: ApplicationFiled: December 13, 2019Publication date: April 2, 2020Inventors: Daniel Farley, Kyriacos Mitrophanous
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Publication number: 20200040361Abstract: Provided is a construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1) and (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC) wherein the nucleotide sequence encoding TH is linked to the nucleotide sequence encoding CH1 such that they encode a fusion protein TH-CH1. Also provided is a construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1) and (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC) wherein the nucleotide sequence encoding AADC is linked to the nucleotide sequence encoding TH such that they encode a fusion protein AADC-TH or TH-AADC. Further provided is a viral vector comprising such nucleotide sequences and its use in the treatment and/or prevention of Parkinson's disease.Type: ApplicationFiled: June 26, 2019Publication date: February 6, 2020Inventors: Kyriacos A. MITROPHANOUS, Scott RALPH, Hannah STEWART, Alan John KINGSMAN
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Patent number: 10544429Abstract: The present invention relates to a nucleic acid sequence comprising a binding site operably linked to a nucleotide of interest, wherein the binding site is capable of interacting with an RNA-binding protein such that translation of the nucleotide of interest is repressed in a viral vector production cell.Type: GrantFiled: December 19, 2014Date of Patent: January 28, 2020Assignee: Oxford BioMedica (UK) LimitedInventors: Daniel Farley, Kyriacos Mitrophanous
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Patent number: 10400252Abstract: Provided is a construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1) and (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC) wherein the nucleotide sequence encoding TH is linked to the nucleotide sequence encoding CH1 such that they encode a fusion protein TH-CH1. Also provided is a construct comprising (i) a nucleotide sequence which encodes tyrosine hydroxylase (TH), (ii) a nucleotide sequence which encodes GTP-cyclohydrolase I (CH1) and (iii) a nucleotide sequence which encodes Aromatic Amino Acid Dopa Decarboxylase (AADC) wherein the nucleotide sequence encoding AADC is linked to the nucleotide sequence encoding TH such that they encode a fusion protein AADC-TH or TH-AADC. Further provided is a viral vector comprising such nucleotide sequences and its use in the treatment and/or prevention of Parkinson's disease.Type: GrantFiled: October 26, 2012Date of Patent: September 3, 2019Assignee: Oxford BioMedica (UK) Ltd.Inventors: Kyriacos A. Mitrophanous, Scott Ralph, Hannah Stewart, Alan John Kingsman
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Publication number: 20190211358Abstract: A cell for producing retroviral vectors comprising nucleic acid sequences encoding: i) gag-pol; ii) env; iii) the RNA genome of the retroviral vector; and iv) optionally rev, or a functional substitute thereof, wherein at least two nucleic acid sequences are located at the same genetic locus; and wherein the at least two nucleic acid sequences are in reverse and/or alternating orientations.Type: ApplicationFiled: December 21, 2018Publication date: July 11, 2019Inventors: Hannah Stewart, Helen Maunder, Kyriacos Mitrophanous, Daniel Farley
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Patent number: 10273502Abstract: A process for producing a retroviral or lentiviral vector formulation comprising a filter-sterilisation step wherein the filter-sterilisation step is not the final step in the purification process.Type: GrantFiled: September 14, 2015Date of Patent: April 30, 2019Assignee: Oxford BioMedica (UK) LimitedInventors: Richard Truran, Robert Buckley, Pippa Radcliffe, James Miskin, Kyriacos Mitrophanous
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Patent number: 9738907Abstract: Provided is a multicistronic retroviral vector genome having a first nucleic acid sequence upstream of at least one internal regulatory element, such that the level of genomic RNA available for packaging in the absence of rev, or a functional equivalent thereof, is increased.Type: GrantFiled: May 7, 2004Date of Patent: August 22, 2017Assignee: Oxford BioMedica (UK) LimitedInventors: Philippa Radcliffe, James E. Miskin, Fraser J. Wilkes, Kyriacos A. Mitrophanous, Susan M. Kingsman
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Publication number: 20170175135Abstract: A process for producing a retroviral or lentiviral vector formulation comprising a filter-sterilisation step wherein the filter-sterilisation step is not the final step in the purification process.Type: ApplicationFiled: September 14, 2015Publication date: June 22, 2017Applicant: OXFORD BIOMEDICA (UK) LIMITEDInventors: Richard Truran, Robert Buckley, Pippa Radcliffe, James Miskin, Kyriacos Mitrophanous
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Publication number: 20170073702Abstract: A process for producing a retroviral or lentiviral vector formulation comprising a filter-sterilisation step wherein the filter-sterilisation step is not the final step in the purification process.Type: ApplicationFiled: September 14, 2015Publication date: March 16, 2017Applicant: OXFORD BIOMEDICA (UK) LIMITEDInventors: Richard Truran, Robert Buckley, Pippa Radcliffe, James Miskin, Kyriacos Mitrophanous
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Publication number: 20160333373Abstract: The present invention relates to a nucleic acid sequence comprising a binding site operably linked to a nucleotide of interest, wherein the binding site is capable of interacting with an RNA-binding protein such that translation of the nucleotide of interest is repressed in a viral vector production cell.Type: ApplicationFiled: December 19, 2014Publication date: November 17, 2016Inventors: Daniel Farley, Kyriacos Mitrophanous
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Patent number: 9339512Abstract: Provided is a lentiviral vector for delivery to the brain for use in treating a neurological condition, wherein the lentiviral vector is delivered directly to the brain by delivering the lentiviral vector via six or fewer tracts per hemisphere, at a single deposit point per tract.Type: GrantFiled: May 14, 2013Date of Patent: May 17, 2016Assignee: Oxford BioMedica (UK) LimitedInventors: Peter Widdowson, Scott Ralph, Kyriacos A. Mitrophanous
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Patent number: 9169491Abstract: A process for producing a retroviral or lentiviral vector formulation comprising a filter-sterilization step wherein the filter-sterilization step is not the final step in the purification process.Type: GrantFiled: June 18, 2009Date of Patent: October 27, 2015Assignee: Oxford BioMedica (UK) LimitedInventors: Richard Truran, Robert Buckley, Pippa Radcliffe, James Miskin, Kyriacos Mitrophanous
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Publication number: 20130281975Abstract: Provided is a lentiviral vector for delivery to the brain for use in treating a neurological condition, wherein the lentiviral vector is delivered directly to the brain by delivering the lentiviral vector via six or fewer tracts per hemisphere, at a single deposit point per tract.Type: ApplicationFiled: May 14, 2013Publication date: October 24, 2013Inventors: Peter WIDDOWSON, Scott Ralph, Kyriacos A. Mitrophanous
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Publication number: 20120295960Abstract: Provided is an improved treatment for Parkinson's Disease where the efficacy of L-Dopa treatment is increased by including gene therapy in the treatment regimen. The combination therapy results in long-term improvements in response to L-Dopa and diminished side effects caused by L-Dopa.Type: ApplicationFiled: January 9, 2012Publication date: November 22, 2012Inventors: Stephane Palfi, Kyriacos A. Mitrophanous, Scott Ralph
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Publication number: 20110293571Abstract: Provided is a lentiviral vector for delivery to the brain for use in treating a neurological condition, wherein the lentiviral vector is delivered directly to the brain by delivering the lentiviral vector via six or fewer tracts per hemisphere, at a single deposit point per tract.Type: ApplicationFiled: May 27, 2011Publication date: December 1, 2011Applicant: Oxford BioMedica (UK) Ltd.Inventors: Peter WIDDOWSON, Scott Ralph, Kyriacos A. Mitrophanous
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Publication number: 20110269826Abstract: The present invention provides methods for: (i) treating and/or preventing Parkinson's disease in a subject without causing cognitive impairment by using dopamine replacement gene therapy to maintain or restore constant physiological dopaminergic tone in both the dorsal and ventral striatum of the subject; (ii) normalising neuronal electrical activity in basal ganglia and/or subthalamic nucleus in a Parkinson's disease subject; and (iii) treating and/or preventing dyskinesias associated with oral L-dopa administration in a Parkinson's disease subject by administration of a vector system for dopamine replacement gene therapy to the subject.Type: ApplicationFiled: November 11, 2009Publication date: November 3, 2011Applicant: OXFORD BIOMEDICA (UK) LIMITEDInventors: Susan M. Kingsman, Alan J. Kingsman, Scott Ralph, Kyriacos A. Mitrophanous, Stephane Palfi, Bechir Jarraya