Abstract: A well plate and a 3D cell culture plate are described. The well plate having a repeating pattern. The wells of the well plate is continuously provided with a space part in between a main well to be injected with culture solution and a sub well including a recessed part where the cells are cultured. The main well of the well plate is connected to the space part with forming a step, and the sub well is connected to the part with forming an inclined surface.

Abstract: A method of producing induced pluripotent stem cells without using a hydrogel.

Abstract: The present invention provides a stem cell mass proliferation method without using a hydrogel.

Abstract: The present invention provides a method of producing brain organoids.

Abstract: The present invention provides a method for isolating mesenchymal stem cells derived from a placental chorionic plate membrane, the method including: (a) harvesting a chorionic plate membrane from a detached placenta; (b) harvesting cells present in the chorionic plate membrane obtained in step (a) by scraping; (c) adding a solution containing trypsin and ethylenediaminetetraacetate to the cells obtained in step (b) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; and (d) centrifuging the reaction solution obtained in step (c) and culturing the obtained cells in a medium containing a fetal bovine serum and an antibiotic.