Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention concerns polynucleotides encoding a small subunit of plant AGP having one or more mutations in the amino acid sequence wherein the mutation confers increased heat stability to the expressed AGP enzyme. Mutations in the N-terminus of the small subunit of heat labile plant AGP results in AGP enzymes that are significantly more heat stable compared to wild type AGP in that the mutant AGP retains significant levels of enzymatic activity following exposure to heat treatment. In one embodiment, the polynucleotide encodes a mutant small subunit of maize AGP. The subject invention also concerns methods for providing a plant with increased resistance to heat conditions. Plants with heat labile AGP can be transformed with a polynucleotide of the present invention. The subject invention also concerns these transformed plants and transgenic progeny thereof. The subject invention also concerns mutant polypeptides encoded by polynucleotides of the present invention.

Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased starch biosynthesis. Increased starch biosynthesis provides for increased yield. One aspect of the invention concerns polynucleotides that encode a mutant plant large subunit of AGPase. In one embodiment, a polynucleotide of the invention encodes a plant AGPase large subunit having an amino acid mutation wherein the cysteine amino acid corresponding to amino acid position 424 of wild type maize AGPase large subunit is substituted with an amino acid that results in increased AGPase activity when provided in an AGPase enzyme. In a specific embodiment, the cysteine corresponding to amino acid position 424 of wild type maize AGPase large subunit is substituted with a valine amino acid. The subject invention also comprises a mutant plant large subunit of AGPase encoded by a polynucleotide of the invention.

Abstract: The subject invention concerns polynucleotides encoding a small subunit of plant AGP having one or more mutations in the amino acid sequence wherein the mutation confers increased heat stability to the expressed AGP enzyme. Mutations in the N-terminus of the small subunit of heat labile plant AGP results in AGP enzymes that are significantly more heat stable compared to wild type AGP in that the mutant AGP retains significant levels of enzymatic activity following exposure to heat treatment. In one embodiment, the polynucleotide encodes a mutant small subunit of maize AGP. The subject invention also concerns methods for providing a plant with increased resistance to heat conditions. Plants with heat labile AGP can be transformed with a polynucleotide of the present invention. The subject invention also concerns these transformed plants and transgenic progeny thereof. The subject invention also concerns mutant polypeptides encoded by polynucleotides of the present invention.

Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased starch biosynthesis. Increased starch biosynthesis provides for increased yield. One aspect of the invention concerns polynucleotides that encode a mutant plant large subunit of AGPase. In one embodiment, a polynucleotide of the invention encodes a plant AGPase large subunit having an amino acid mutation wherein the cysteine amino acid corresponding to amino acid position 424 of wild type maize AGPase large subunit is substituted with an amino acid that results in increased AGPase activity when provided in an AGPase enzyme. In a specific embodiment, the cysteine corresponding to amino acid position 424 of wild type maize AGPase large subunit is substituted with a valine amino acid. The subject invention also comprises a mutant plant large subunit of AGPase encoded by a polynucleotide of the invention.

Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention concerns chimeric AGP subunit proteins and polynucleotides that encode the chimeric proteins. The subject invention provides for mutant AGP enzymes comprising a chimeric subunit of the invention that are less sensitive to inorganic phosphate than wild type AGP enzymes. In one embodiment, the AGP subunit is a small subunit of a plant AGP enzyme. The subject invention also concerns plants comprising a polynucleotide encoding a chimeric AGP subunit protein of the invention. The subject invention also concerns methods for producing a plant comprising a polynucleotide of the present invention. Plants produced according to the invention comprise AGP enzymes that are less sensitive to inorganic phosphate than wild type AGP enzyme.

Abstract: The subject invention pertains to novel mutant polynucleolide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. In one embodiment, the polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) activity. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions or heat stress are also provided.

Abstract: The subject invention concerns materials and methods for enhancing starch production in plants. Starch production is enhanced, relative to levels observed in wildtype or control plants, by reduction of the plant 14-3-3 protein(s) which subsequently results in increased accumulation of starch in the plant. In one embodiment, the 14-3-3 protein expression is reduced using polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant. In another embodiment, the 14-3-3 protein expression is reduced by “knockout” of a 14-3-3 gene or gene sequences. The subject invention also pertains to transformed and transgenic plants that have polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant, wherein the transformed and transgenic plants exhibit enhanced starch production. The subject invention also pertains to “knockout” plants in which the normal functional 14-3-3 gene in the plant is deleted or replaced with a non-functional form of the gene.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention concerns chimeric AGP subunit proteins and polynucleotides that encode the chimeric proteins. The subject invention provides for mutant AGP enzymes comprising a chimeric subunit of the invention that are less sensitive to inorganic phosphate than wild type AGP enzymes. In one embodiment, the AGP subunit is a small subunit of a plant AGP enzyme. The subject invention also concerns plants comprising a polynucleotide encoding a chimeric AGP subunit protein of the invention. The subject invention also concerns methods for producing a plant comprising a polynucleotide of the present invention. Plants produced according to the invention comprise AGP enzymes that are less sensitive to inorganic phosphate than wild type AGP enzyme.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. In one embodiment, the polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activties. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

Abstract: The subject invention concerns materials and methods for enhancing starch production in plants. Starch production is enhanced, relative to levels observed in wildtype or control plants, by reduction of the plant 14-3-3 protein(s) which subsequently results in increased accumulation of starch in the plant. In one embodiment, the 14-3-3 protein expression is reduced using polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant. In another embodiment, the 14-3-3 protein expression is reduced by “knockout” of a 14-3-3 gene or gene sequences. The subject invention also pertains to transformed and transgenic plants that have polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant, wherein the transformed and transgenic plants exhibit enhanced starch production.