Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: The present invention provides compositions that inhibit apoptosis, methods for making the compositions, and methods of use thereof.

Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: The present invention is to a method of screening agents for potential therapeutic efficacy. The method comprises exposing a cell strain that is sensitive to apoptotic agents to known apoptotic agents and to a potential therapeutic agent. The cell strain is then cultured and cells are removed that display diminished adherence. The remaining, adherent, cells are then incubated in the presence of a proteinase to release proteinase sensitive cells. The proteinase sensitive cells are removed to yield proteinase resistant cells and the proteinase sensitive cells are counted. The proteinase resistant cells are then collected and counted. The agent is determined to have potential therapeutic efficacy if the ratio of proteinase sensitive cells to proteinase resistant cells changes relative to a control as a result of the presence of the agent.

Abstract: The present invention is to a method of screening agents for potential therapeutic efficacy. The method comprises exposing a cell strain that is sensitive to apoptotic agents to known apoptotic agents and to a potential therapeutic agent. The cell strain is then cultured and cells are removed that display diminished adherence. The remaining, adherent, cells are then incubated in the presence of a proteinase to release proteinase sensitive cells. The proteinase sensitive cells are removed to yield proteinase resistant cells and the proteinase sensitive cells are counted. The proteinase resistant cells are then collected and counted. The agent is determined to have potential therapeutic efficacy if the ratio of proteinase sensitive cells to proteinase resistant cells changes relative to a control as a result of the presence of the agent.

Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: The present invention is directed to methods of obtaining plant-derived delipidated extracts that inhibit apoptosis, the extracts obtained, compositions containing said extracts and methods of using said compositions. FIG. 11 is a bar graph which illustrates a lower incidence of diarrhea in rats treated with methotrexate and fed a diet of compositions of the claimed invention as compared to controls.

Abstract: The present invention is directed to methods of obtaining plant-derived delipidated extracts that inhibit apoptosis, the extracts obtained, compositions containing said extracts and methods of using said compositions. The figure is a bar graph which illustrates a lower incidence of diarrhea in rats treated with methotrexate and fed a diet of compositions of the claimed invention as compared to controls.

Abstract: The present invention is to methods of obtaining plant-derived compositions that-inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: The present invention provides cell strains suitable for use in screening agents for potential therapeutic efficacy. The cell strains retain responsiveness to apoptosis inducers and inhibitors.

Abstract: The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.

Abstract: A laser imaging system is disclosed which provides the versatility of wide field digital imaging with enhanced spatial resolution and light gathering efficiency. The system will scan targets of any size, dependent only upon the data retrieval and storage limitation of the computer support system, for forward light loss densitometry images as well as fluorescent and forward scatter images. The system is easily adaptable for rare event detection and tracking. The laser system will provide image capture of an entire target within 10 to 60 seconds and controls the scan of the laser beam in three-dimensional pattern and speed. The beam may be repositioned to any one of 16 million locations on a target within an accuracy of +/-0.5 um. Finally, the imaging system of the present invention utilizes a novel optical fiber based detector assembly having NA values of 0.58-0.95 and filters having less than 15% loss at emission wavelengths.

Abstract: A method and apparatus for measuring low-level laser-induced fluorescence are disclosed. A laser is used to produce a coherent beam of light which is sequentially passed through a beam expander, iris diaphram, focusing lens and three-dimensional scanner before being focused onto a rigidly mounted target. A computer is used to predeterminately control the pattern and the rate at which the scanner passes the beam of light over the target. The light transmitted onto the target induces fluorescent light in the target. The fluorescent light is sequentially gathered by a biased cut optical fiber member and directed into a photomultiplier tube where the intensity of the fluorescent light is measured. The intensity data is then digitized and recorded by the computer as a function of the coordinates of each preprogrammed point location of the beam impinging upon the target. This data is used to produce an image of all or a portion of the target on a visual monitor.

Abstract: A method and apparatus for measuring low-level laser-induced fluorescence are disclosed. A laser is used to produce a coherent beam of light which is sequentially passed through a beam expander, iris diaphram, focusing lens and three-dimensional scanner before being focused onto a rigidly mounted target. A computer is used to predeterminately control the pattern and the rate at which the scanner passes the beam of light over the target. The light transmitted onto the target induces fluorescent light in the target. The fluorescent light is sequentially gathered by a biased cut optical fiber member and directed into a photomultiplier tube where the intensity of the fluorescent light is measured. The intensity data is then digitized and recorded by the computer as a function of the coordinates of each preprogrammed point location of the beam impinging upon the target. This data is used to produce an image of all or a portion of the target on a visual monitor.

Abstract: A viral vaccine effective against foot-and-mouth disease is produced without the use of serum or non-chemically defined serum substitutes. A viable culture of baby hamster kidney cells in a heat-stable, glutamine-free, serum-free, chemically defined medium is inoculated with one of the seven major immunological types of foot-and-mouth disease viruses to which the cells are susceptible, the virus is propagated in the culture and the virus harvest is recovered therefrom.