Patents by Inventor Lakshmi Rambhatla
Lakshmi Rambhatla has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9587223Abstract: This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.Type: GrantFiled: August 27, 2013Date of Patent: March 7, 2017Assignee: Asterias Biotherapeutics, Inc.Inventors: Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau, Kristina Pfendler-Bonham, Namitha Rao, Melissa K. Carpenter, Lakshmi Rambhatla, Choy-Pik Chiu
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Publication number: 20140106343Abstract: This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.Type: ApplicationFiled: August 27, 2013Publication date: April 17, 2014Applicant: Asterias Biotherapeutics, Inc.Inventors: Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau, Kristina Pfendler-Bonham, Namitha Rao, Melissa K. Carpenter, Lakshmi Rambhatla, Choy-Pik Chiu
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Patent number: 8546139Abstract: This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.Type: GrantFiled: November 24, 2008Date of Patent: October 1, 2013Assignee: Geron CorporationInventors: Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau, Kristina Pfendler-Bonham, Namitha Rao, Melissa K. Carpenter, Lakshmi Rambhatla, Choy-Pik Chiu
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Publication number: 20090136955Abstract: This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.Type: ApplicationFiled: November 24, 2008Publication date: May 28, 2009Inventors: Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau, Kristina Pfendler-Bonham, Namitha Rao, Melissa K. Carpenter, Lakshmi Rambhatla, Choy-Pik Chiu
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Patent number: 7473555Abstract: This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.Type: GrantFiled: March 26, 2004Date of Patent: January 6, 2009Assignee: Geron CorporationInventors: Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau, Kristina Pfendler-Bonham, Namitha Rao, Melissa K. Carpenter, Lakshmi Rambhatla, Choy-Pik Chiu
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Patent number: 7282366Abstract: It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function.Type: GrantFiled: March 1, 2002Date of Patent: October 16, 2007Assignee: Geron CorporationInventors: Lakshmi Rambhatla, Melissa K. Carpenter
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Patent number: 7256042Abstract: It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function.Type: GrantFiled: October 31, 2001Date of Patent: August 14, 2007Assignee: Geron CorporationInventors: Lakshmi Rambhatla, Melissa K. Carpenter
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Publication number: 20050037493Abstract: This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.Type: ApplicationFiled: March 26, 2004Publication date: February 17, 2005Inventors: Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau, Kristina Pfendler-Bonham, Namitha Rao, Melissa Carpenter, Lakshmi Rambhatla, Choy-Pik Chiu
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Patent number: 6506574Abstract: It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function.Type: GrantFiled: May 31, 2001Date of Patent: January 14, 2003Assignee: Geron CorporationInventors: Lakshmi Rambhatla, Melissa K. Carpenter
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Publication number: 20020160511Abstract: It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function.Type: ApplicationFiled: October 31, 2001Publication date: October 31, 2002Inventors: Lakshmi Rambhatla, Melissa K. Carpenter
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Patent number: 6458589Abstract: It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function.Type: GrantFiled: November 20, 2000Date of Patent: October 1, 2002Assignee: Geron CorporationInventors: Lakshmi Rambhatla, Melissa K. Carpenter