Patents by Inventor Lalit Chander Garg

Lalit Chander Garg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Recombinant vaccine against infection and epsilon toxin intoxication
    Patent number: 9408900
    Abstract: The present invention relates to generation and high level expression of recombinant non-toxic of epsilon toxin of Clostridium perfringens as a recombinant vaccine against Clostridium perfringens infection and a process for producing the vaccine involving amplifying, cloning, transforming, incubating and purifying the recombinant non-toxic epsilon toxin protein. Thus in this invention, substitution mutation Y71G was executed in recombinant Etx and the recombinant EtxY71G protein was over-expressed in soluble form. Expressed protein was purified near homogeneity by DEAE sepharose anion exchange chromatography with high yield. Potential of rEtxY71G as a vaccine candidate was evaluated and found to be highly specific and immunogenic. The present invention is the first report for high level expression of non toxic rEtxY71G mutant protein of Clostridium perfringens. Upto 100 mg/L of highly immunogenic and homogeneous recombinant EtxY71G protein of 31 kDa was produced.
    Type: Grant
    Filed: July 1, 2011
    Date of Patent: August 9, 2016
    Assignee: National Institute of Immunology
    Inventors: Lalit Chander Garg, Keshav Gopal, Aparna Dixit
  • RECOMBINANT VACCINE AGAINST CLOSTRIDIUM PERFRINGENS INFECTION AND EPSILON TOXIN INTOXICATION
    Publication number: 20130183344
    Abstract: The present invention relates to generation and high level expression of recombinant non-toxic of epsilon toxin of Clostridium perfringens as a recombinant vaccine against Clostridium perfringens infection and a process for producing the vaccine involving amplifying, cloning, transforming, incubating and purifying the recombinant non-toxic epsilon toxin protein. Thus in this invention, substitution mutation Y71G was executed in recombinant Etx and the recombinant EtxY71G protein was over-expressed in soluble form. Expressed protein was purified near homogeneity by DEAE sepharose anion exchange chromatography with high yield. Potential of rEtxY71G as a vaccine candidate was evaluated and found to be highly specific and immunogenic. The present invention is the first report for high level expression of non toxic rEtxY71G mutant protein of Clostridium perfringens. Upto 100 mg/L of highly immunogenic and homogeneous recombinant EtxY71G protein of 31 kDa was produced.
    Type: Application
    Filed: July 1, 2011
    Publication date: July 18, 2013
    Applicant: NATIONAL INSTITUTE OF IMMUNOLOGY
    Inventors: Lalit Chander Garg, Keshav Gopal, Aparna Dixit
  • Process for solubilization of recombinant proteins expressed as inclusion body
    Patent number: 7189811
    Abstract: The present invention relates to the solubilization and recovery in high yield, of inclusion body proteins from host cells using an appropriate denaturating agent. The process avoids the use of high concentration of chaotropic agents such as guanidine hydrochloride or urea.
    Type: Grant
    Filed: September 5, 2003
    Date of Patent: March 13, 2007
    Assignee: National Institute of Immunology
    Inventors: Amulya Kumar Panda, Mohammed Gulebahar Sheikh, Addala Naga Sai Eshwari, Lalit Chander Garg
  • Process for solubilization of recombinant proteins expressed as inclusion body
    Publication number: 20040235089
    Abstract: The present invention relates to the solubilization and recovery in high yield, of inclusion body proteins from host cells using an appropriate denaturating agent. The process avoids the use of high concentration of chaotropic agents such as guanidine hydrochloride or urea.
    Type: Application
    Filed: September 5, 2003
    Publication date: November 25, 2004
    Inventors: Amulya Kumar Panda, Mohammed Gulebahar Sheikh, Addala Naga Sai Eshwari, Lalit Chander Garg