Abstract: A light diffusing device for a charger with smart night light is provided, including a light-emitting component, a sensor light disposed on the light-emitting component, a trumpet-shaped reflector and a light-permeable upper housing. The reflector is provided on the light-emitting component, the sensor light is disposed in a trumpet-shaped interior chamber of the reflector, LED lamp beads are disposed on the light-emitting component and surround the sensor light, lights from the LED lamp beads are emitted to a rear face of the reflector, and the light-permeable upper housing is provided on the light-emitting component and the reflector. Light emitted from each LED lamp bead is a point light source and a direct light, and is scattered by the rear face of the reflector and then projected through the upper housing, such that the irradiation range is widened and the light is diffused towards the surroundings.

Abstract: The present invention provides a method for analyzing simultaneously multiple human antigen-specific cell populations of a sample, the sample comprising B cells and antigen-specific cells, the method comprising a) separation of B cells from said sample, b) dividing the B cells into n sub-samples, c) differentially labeling the B cells of said sub-samples, wherein at least n-1sub-samples are labeled, d) pulsing of the B cells of each sub-sample with single or multiple peptides, e) pooling of the labeled and peptide-pulsed B cells with cells of said sample comprising said antigen-specific cells, f) co-cultivation of the cells of step e), g) flow cytometry analysis of the B cells with regard to their cell number and CD83 expression, thereby determining the potency of said antigen-specific cells in said sample.

Abstract: The present invention provides a method for analyzing simultaneously multiple human antigen-specific cell populations of a sample, the sample comprising B cells and antigen-specific cells, the method comprising a) separation of B cells from said sample, b) dividing the B cells into n sub-samples, c) differentially labeling the B cells of said sub-samples, wherein at least n-1sub-samples are labeled, d) pulsing of the B cells of each sub-sample with single or multiple peptides, e) pooling of the labeled and peptide-pulsed B cells with cells of said sample comprising said antigen-specific cells, f) co-cultivation of the cells of step e), g) flow cytometry analysis of the B cells with regard to their cell number and CD83 expression, thereby determining the potency of said antigen-specific cells in said sample.