Abstract: An imaging system includes a housing assembly having a head unit configured to be at least partially directed towards a target site. An optical coherence tomography (OCT) module and a visualization module are located in the housing assembly and configured to respectively obtain OCT data and visualization data of the target site. The system includes a controller configured to generate a scanning pattern for a region of calibration selected in a calibration target. OCT data of the region of calibration is synchronously acquired. The controller is configured to obtain a projected two-dimensional OCT image of the region of calibration based on the OCT data, as an inverse mean-intensity projection. The controller is configured to register the projected two-dimensional OCT image to a corresponding view extracted from the visualization data, via a cascaded image registration process having a coarse registration stage and a fine registration stage.

Abstract: An apparatus and method of use are disclosed to automatically process and transfer fluids. The apparatus may include a user-interface, clamps, pumps, spinning membranes, pressure transducers, fluid detectors, solution towers, weight scales and tubing sets to control and monitor cell washing, concentrating and harvesting procedures. In addition, the apparatus may also be used to control and monitor a variety of fluid transfer procedures. The apparatus may also be used to execute default or user-defined cell washing and fluid transfer procedures.