Abstract: Methods and systems to produce product compositions comprising caprylate products using chain-elongating bacteria. For example, the caprylate product in the product composition is n-caprylic acid (C8) and the n-caprylic (C8) to n-caproic (C6) acid ratio is higher than 1:1. These methods use chain elongation towards C8 rather than C6. High n-caprylate productivity and specificity was accomplished by: 1) feeding a substrate with, for example, ethanol as the carbon source or alternatively, a high ethanol-to-acetate ratio as the carbon source; 2) extracting caprylate product(s) (e.g., n-caprylate product) from the bioreactor broth; and 3) acclimating an efficient chain-elongating microbiome. The methods can produce caprylate products such as, for example, n-caprylic acid, which is a higher value chemical than C4 and C6.

Abstract: Methods and systems to produce product compositions comprising caprylate products using chain-elongating bacteria. For example, the caprylate product in the product composition is n-caprylic acid (C8) and the n-caprylic (C8) to n-caproic (C6) acid ratio is higher than 1:1. These methods use chain elongation towards C8 rather than C6. High n-caprylate productivity and specificity was accomplished by: 1) feeding a substrate with, for example, ethanol as the carbon source or alternatively, a high ethanol-to-acetate ratio as the carbon source; 2) extracting caprylate product(s) (e.g., n-caprylate product) from the bioreactor broth; and 3) acclimating an efficient chain-elongating microbiome. The methods can produce caprylate products such as, for example, n-caprylic acid, which is a higher value chemical than C4 and C6.

Abstract: Methods and systems to produce product compositions comprising caprylate products using chain-elongating bacteria. For example, the caprylate product in the product composition is n-caprylic acid (C8) and the n-caprylic (C8) to n-caproic (C6) acid ratio is higher than 1:1. These methods use chain elongation towards C8 rather than C6. High n-caprylate productivity and specificity was accomplished by: 1) feeding a substrate with, for example, ethanol as the carbon source or alternatively, a high ethanol-to-acetate ratio as the carbon source; 2) extracting caprylate product(s) (e.g., n-caprylate product) from the bioreactor broth; and 3) acclimating an efficient chain-elongating microbiome. The methods can produce caprylate products such as, for example, n-caprylic acid, which is a higher value chemical than C4 and C6.

Abstract: Methods and systems to produce product compositions comprising caprylate products using chain-elongating bacteria. For example, the caprylate product in the product composition is n-caprylic acid (C8) and the n-caprylic (C8) to n-caproic (C6) acid ratio is higher than 1:1. These methods use chain elongation towards C8 rather than C6. High n-caprylate productivity and specificity was accomplished by: 1) feeding a substrate with, for example, ethanol as the carbon source or alternatively, a high ethanol-to-acetate ratio as the carbon source; 2) extracting caprylate product(s) (e.g., n-caprylate product) from the bioreactor broth; and 3) acclimating an efficient chain-elongating microbiome. The methods can produce caprylate products such as, for example, n-caprylic acid, which is a higher value chemical than C4 and C6.

Abstract: Methods and systems for producing and removing C6 and/or C8 carboxylates and/or methane from carbohydrate containing biomass, an alcohol, and mixtures of microorganisms under an anaerobic environment. The C6 and/or C8 carboxylates are removed continuously or in-line. Methanogenesis is not inhibited and very little input carbon is lost as carbon dioxide.

Abstract: Methods for obtaining a product comprising a substituted or unsubstituted C3 to C10 alcohol from a substituted or unsubstituted C3 to C10 carboxylic acid. The method comprises contacting a substituted or unsubstituted C3 to C10 carboxylic acid with a gaseous composition and a Chlostridia species to produce a substituted or unsubstituted C3 to C10 alcohol under an anaerobic environment.

Abstract: Methods and systems for producing and removing C6 and/or C8 carboxylates and/or methane from carbohydrate containing biomass, an alcohol, and mixtures of microorganisms under an anaerobic environment. The C6 and/or C8 carboxylates are removed continuously or in-line. Methanogenesis is not inhibited and very little input carbon is lost as carbon dioxide.

Abstract: A continuously fed, compartmentalized reactor that reverses its flow in a horizontal manner. This system is developed without the requirement of elaborate gas-solids-separator and feed-distribution systems. Effluent recycling is not required, but mixing is necessary to obtain a sufficient biomass/substrate contact. This process is known as the anaerobic migrating blanket reactor (AMBR). A key to the selection of a granular biomass in the AMBR process, and thus to the reactor performance, is the migration of the blanket through the reactor. A higher rate of migration of flocculent biomass, compared with granular biomass, is responsible for the wash out of less settleable, flocculent biomass. In this way, the formed aggregates are retained in the reactor and grow in size.