Patents by Inventor Larry A. McReynolds
Larry A. McReynolds has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10597650Abstract: Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.Type: GrantFiled: March 14, 2013Date of Patent: March 24, 2020Assignee: New England Biolabs, Inc.Inventors: Gregory Lohman, Thomas C. Evans, Larry A. McReynolds
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Patent number: 10597710Abstract: Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.Type: GrantFiled: August 28, 2015Date of Patent: March 24, 2020Assignee: New England Biolabs, Inc.Inventors: Gregory Lohman, Thomas C. Evans, Jr., Larry A. McReynolds
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Patent number: 9650667Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.Type: GrantFiled: October 7, 2014Date of Patent: May 16, 2017Assignee: New England Biolabs, Inc.Inventors: Larry A. McReynolds, Daniela Munafo
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Publication number: 20160002716Abstract: Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.Type: ApplicationFiled: August 28, 2015Publication date: January 7, 2016Inventors: Gregory Lohman, Thomas C. Evans, JR., Larry A. McReynolds
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Publication number: 20150072870Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.Type: ApplicationFiled: October 7, 2014Publication date: March 12, 2015Applicant: New England Biolabs, Inc.Inventors: Larry A. McReynolds, Daniela Munafo
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Patent number: 8883421Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.Type: GrantFiled: August 31, 2011Date of Patent: November 11, 2014Assignee: New England Biolabs, Inc.Inventors: Larry A. McReynolds, Daniela Munafo
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Patent number: 8753809Abstract: Methods and compositions are provided for detecting small target RNAs where the target RNA may be single-stranded or double-stranded and may be contained in a mixture of RNAs of different types and sizes. The methods and compositions utilize a p19 fusion protein that is capable of binding double-stranded RNA in a size-specific but sequence-independent manner and is further capable of binding to a matrix such as beads or plastic microwell plates. By labeling the p19 fusion protein or the target RNA in a polynucleotide duplex either directly or indirectly, low levels of target RNA including microRNAs can be detected from cells. This can be applied to diagnosis of pathological conditions.Type: GrantFiled: December 21, 2012Date of Patent: June 17, 2014Assignee: New England Biolabs, Inc.Inventors: Larry A. McReynolds, Jingmin Jin
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Publication number: 20130157869Abstract: Methods are provided for ligating a 3? adapter and a 5? adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3? adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3? adapter and the ligated 3? adapter. Subsequently, a 5? adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.Type: ApplicationFiled: August 31, 2011Publication date: June 20, 2013Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Larry A. McReynolds, Daniela Munafo
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Publication number: 20130143276Abstract: A method is provided for generating a preparation in which more than 70% of the oligonucleotides are adenylated. The method includes reacting an oligonucleotide with an ATP-sensitive ligase where the ligase is characterized by its ability to efficiently generate adenylated oligonucleotides at ATP concentrations at which ligation and circularization of the oligonucleotide is minimal.Type: ApplicationFiled: April 1, 2011Publication date: June 6, 2013Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Alexander Zhelkovsky, Larry A. McReynolds
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Publication number: 20100209933Abstract: Methods and compositions are provided for detecting small target RNAs where the target RNA may be single-stranded or double-stranded and may be contained in a mixture of RNAs of different types and sizes. The methods and compositions utilize a p19 fusion protein that is capable of binding double-stranded RNA in a size-specific but sequence-independent manner and is further capable of binding to a matrix such as beads or plastic microwell plates. By labeling the p19 fusion protein or the target RNA in a polynucleotide duplex either directly or indirectly, low levels of target RNA including microRNAs can be detected from cells. This can be applied to diagnosis of pathological conditions.Type: ApplicationFiled: October 29, 2008Publication date: August 19, 2010Inventors: Larry A. McReynolds, Jingmin Jin
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Patent number: 7700758Abstract: A method for obtaining a mixture of heterogenous short double-stranded RNA molecules suitable for use in gene silencing (hsiRNA) by subjecting large double-stranded RNA to enzymatic cleavage under specified conditions. The resulting mixture consistently includes enhanced representation of fragments having a size of 21-22 nucleotides absent any fractionation step. The fragments contain sequences that collectively span the entire length of the large double-stranded RNA from which they are derived. Double-stranded RNA with sequences that individually represent segments of a target mRNA may be analyzed using the methods described herein to identify the most active subset of hsiRNA fragments or individual siRNA fragments for achieving gene silencing for any gene or transcribed sequences. A method is additionally provided for preparing and cloning DNA encoding selected siRNA, hsiRNA mixtures or hairpin sequences to provide a continuous supply of a gene silencing reagent derived from any long double-stranded RNA.Type: GrantFiled: July 18, 2003Date of Patent: April 20, 2010Assignee: New England Biolabs, Inc.Inventors: George Tzertzinis, George Feehery, Christopher Noren, Corinna Tuckey, Larry McReynolds, Yinhua Zhang
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Publication number: 20080206835Abstract: A method for obtaining a mixture of heterogenous short double-stranded RNA molecules suitable for use in gene silencing (hsiRNA) by subjecting large double-stranded RNA to enzymatic cleavage under specified conditions. The resulting mixture consistently includes enhanced representation of fragments having a size of 21-22 nucleotides absent any fractionation step. The fragments contain sequences that collectively span the entire length of the large double-stranded RNA from which they are derived. Double-stranded RNA with sequences that individually represent segments of a target mRNA may be analyzed using the methods described herein to identify the most active subset of hsiRNA fragments or individual siRNA fragments for achieving gene silencing for any gene or transcribed sequences. A method is additionally provided for preparing and cloning DNA encoding selected siRNA, hsiRNA mixtures or hairpin sequences to provide a continuous supply of a gene silencing reagent derived from any long double-stranded RNA.Type: ApplicationFiled: February 11, 2008Publication date: August 28, 2008Applicant: New England Biolabs, Inc.Inventors: George Tzertzinis, George Feehery, Corinna Tuckey, Christopher Noren, Larry McReynolds, Yinhua Zhang
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Publication number: 20070099234Abstract: Methods and kits are provided for detecting chitin in biological samples using chitin-binding domain (CBD).Type: ApplicationFiled: June 7, 2004Publication date: May 3, 2007Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Yinhua Zhang, Larry McReynolds
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Publication number: 20040038278Abstract: A method for obtaining a mixture of heterogenous short double-stranded RNA molecules suitable for use in gene silencing (hsiRNA) by subjecting large double-stranded RNA to enzymatic cleavage under specified conditions. The resulting mixture consistently includes enhanced representation of fragments having a size of 21-22 nucleotides absent any fractionation step. The fragments contain sequences that collectively span the entire length of the large double-stranded RNA from which they are derived. Double-stranded RNA with sequences that individually represent segments of a target mRNA may be analyzed using the methods described herein to identify the most active subset of hsiRNA fragments or individual siRNA fragments for achieving gene silencing for any gene or transcribed sequences. A method is additionally provided for preparing and cloning DNA encoding selected siRNA, hsiRNA mixtures or hairpin sequences to provide a continuous supply of a gene silencing reagent derived from any long double-stranded RNA.Type: ApplicationFiled: July 18, 2003Publication date: February 26, 2004Inventors: George Tzertzinis, George Feehery, Corinna Tuckey, Christopher Noren, Larry McReynolds
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Patent number: 5350959Abstract: An electrical connection for connecting the interior of an alternating current generator to the exterior of the generator. The generator has a three phase full-wave bridge rectifier that has three AC input terminal studs. The ends of three power supply conductors are connected to the terminal studs by terminals. The conductors extend from the terminal studs to the exterior of the generator and they extend through an opening formed in a side wall of the frame. The conductors are supported and guided by a conductor support part that is formed of plastic material. This conductor support part has a clamping block portion and a U-shaped conductor support portion. Portions of the conductors are disposed between a clamping block and the clamping block portion of the conductor support part. A plurality of threaded fasteners extend through aligned openings in the clamping block and in the block portion and are threaded into threaded bores formed in a lug portion of the end frame.Type: GrantFiled: May 20, 1993Date of Patent: September 27, 1994Assignee: General Motors CorporationInventors: Keith F. Flaminio, Larry McReynolds