Abstract: The present invention provides platelet production methods. The method comprises the steps of providing a cellular material and culturing the cellular material, wherein platelets are produced. The culturing may be performed on 2D or 3D cell support structure or in suspension culture. In some embodiments, all or a part of the 2D or 3D culturing may be performed in a bioreactor. In some embodiments, the method may further comprise a step of isolating a subset of cells from the starting cellular material, wherein the isolated subset of cells is then cultured, wherein platelets are produced. In yet other embodiments, the method comprises the steps of providing a cellular material, isolating a subset of cells, seeding the subset of cells into a 3D scaffold, culturing the subset of cells in a 3D scaffold, seeding the cultured subset of cells into a bioreactor, culturing the subset of cells in a bioreactor, and harvesting the cells from the bioreactor, wherein platelets are produced.