Abstract: The disclosure provides methods and related kits, reagents, and systems for selectively deaminating unmethylated cytosine residues in nucleic acid molecules. In some embodiments, the methods and related kits, reagents, and systems are applied for methods of detecting and/or mapping methylated cytosine residues in nucleic acids. The nucleic can be RNA or DNA. Some embodiments include contacting the polynucleic acid with a bacterial cytosine deaminase, for example DddA or SsdA, or functional fragments or derivatives thereof. Representative DddA and SsdA have sequences set forth in SEQ ID NOS:1 and 2, respectively. The bacterial cytosine deaminases of the disclosure are sensitive to methylation and, thus, deaminate only unmethylated cytosines to provide a cytosine to uracil conversion. The conversion can be detected as a C•G-to-T•A transitions in subsequent sequencing analysis.

Abstract: An aircraft load cell shoring for placing a wing with an aircraft engine attached to the wing in an unloaded condition and for use with a load cell mounted on an aircraft jack. The aircraft load cell shoring includes a load cell adapter and a shoring contour assembly. The load cell adapter is able to be disposed above the load cell. The shoring contour assembly is an assembly for supporting an aircraft engine, and has a shoring contour assembly bottom portion, and a fixed receptacle located at the shoring contour assembly bottom portion. The load cell adapter communicates with the fixed receptacle. The fixed receptacle is located such that the aircraft jack is aligned with the center of gravity of the engine and enclosed part of the wing.