Abstract: A cuvette ampule for use with automated chemical and microbiological analysis apparatuses. The nutrient containing ampule features an upper detachable stem and lower body portion which includes a pair of flat, uniformly spaced sides having the desired optical characteristics. One or more of the cuvette ampules is to be seated and maintained in a fixed position within a cartridge to facilitate insertion into analytical apparatuses. Breaking and removing the ampule stem provides an opening for receipt of the chemical or biological fluid to be evaluated. A compressible member covers each opening to seal the opened ampule and maintain the position of the ampule in the cartridge.