Patents by Inventor Larry Gentry

Larry Gentry has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cloning and expression of simian transforming growth factor .beta.1
    Patent number: 5844085
    Abstract: Recombinant transforming growth factor-.beta.1 (TGF-.beta.1) is expressed to high levels in Chinese hamster ovary (CHO) cells using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-.beta.1 and mouse dhfr cDNAs. Transcription of both cDNAs was controlled by the SV40 early promoter. Stepwise selection of transfected CHO cells in increasing concentrations of methotrexate yielded cell lines expressing amplified TGF-.beta.1 nucleic acid sequences. The expression plasmid DNA was amplified greater than 35-fold in one of the methotrexate selected transfectants. The major proteins secreted by these cells consisted of latent TGF-.beta.1 and TGF-.beta.1 precursor polypeptides as judged by immunoblots using site-specific anti-peptide antibodies derived from various regions of the TGF-.beta.1 precursor. Levels of recombinant TGF-.beta.1 protein secreted by these cells approached 30 ug/24 hour/10.sup.7 cells.
    Type: Grant
    Filed: October 8, 1992
    Date of Patent: December 1, 1998
    Assignee: Bristol-Myers Squibb Pharmaceutical Research Institute-Seattle
    Inventors: Anthony F. Purchio, Larry Gentry, Daniel Twardzik, Amy M. Brunner