Abstract: The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.

Abstract: The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.