Patents by Inventor Larry J. Kricka
Larry J. Kricka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20110152116Abstract: The present invention provides assays of nanometer-level dimension.Type: ApplicationFiled: February 24, 2011Publication date: June 23, 2011Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIAInventors: Larry J. Kricka, Jason Y. Park
-
Publication number: 20110124965Abstract: Provided are methods for detecting a disease or a condition in a subject, which disease or condition is known to be characterized by abnormal expression of an enzyme The present methods comprise contacting tissue of the subject with a substrate for the enzyme, the substrate chemiluminescing upon reaction with the enzyme, detecting chemiluminescence in the tissue, and, correlating the chemiluminescence to at least one of the presence or absence of the disease or condition in the subject, the stage of the disease or condition in the subject, or the response of the disease or condition in the subject to a therapy regimen The present invention permits sampling, diagnosis, and surveillance of conditions and diseases that are known to be characterized by abnormal expression of at least one enzyme.Type: ApplicationFiled: May 7, 2009Publication date: May 26, 2011Inventors: Jason Y. Park, Larry J. Kricka
-
Patent number: 7919019Abstract: The present invention relates to nanostructures for use in luminescent devices.Type: GrantFiled: April 26, 2006Date of Patent: April 5, 2011Assignee: The Trustees Of The University Of PennsylvaniaInventors: Jason Y Park, Larry J Kricka
-
Patent number: 7892819Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction, chamber, having at least one cross-sectional dimension of about 0.1 to 1000 ?m. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation.Type: GrantFiled: August 11, 2003Date of Patent: February 22, 2011Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Publication number: 20110020876Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction, chamber, having at least one cross-sectional dimension of about 0.1 to 1000 ?m. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation.Type: ApplicationFiled: August 11, 2003Publication date: January 27, 2011Inventors: Peter Wilding, Larry J. Kricka
-
Patent number: 7771985Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: GrantFiled: April 4, 2007Date of Patent: August 10, 2010Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
-
Publication number: 20090246888Abstract: The present invention provides assays of nanometer-level dimension.Type: ApplicationFiled: April 26, 2006Publication date: October 1, 2009Applicant: The Trustees of the University of PennsylvaniaInventors: Larry J. Kricka, Jason Y. Park
-
Patent number: 7494770Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber).Type: GrantFiled: April 30, 2003Date of Patent: February 24, 2009Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Publication number: 20080286856Abstract: The present invention relates to nanostructures for use in luminescent devices.Type: ApplicationFiled: April 26, 2006Publication date: November 20, 2008Applicant: The Trustees of the University of PennsylvaniaInventors: Jason Y. Park, Larry J. Kricka
-
Patent number: 7018830Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: GrantFiled: January 21, 2003Date of Patent: March 28, 2006Assignee: The Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
-
Patent number: 7005292Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: GrantFiled: August 22, 2003Date of Patent: February 28, 2006Assignee: The Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
-
Patent number: 6953676Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber).Type: GrantFiled: December 15, 1998Date of Patent: October 11, 2005Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Patent number: 6660517Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction chamber, having at least one cross-sectional dimension of about 0.1 to 1000 &mgr;m. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation.Type: GrantFiled: November 8, 1996Date of Patent: December 9, 2003Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Publication number: 20030199081Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber).Type: ApplicationFiled: April 30, 2003Publication date: October 23, 2003Inventors: Peter Wilding, Larry J. Kricka
-
Publication number: 20030129671Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: ApplicationFiled: January 21, 2003Publication date: July 10, 2003Inventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
-
Patent number: 6551841Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: GrantFiled: January 27, 1999Date of Patent: April 22, 2003Assignee: The Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
-
Patent number: 6184029Abstract: A mesoscale sample preparation device capable of providing microvolume test samples, separated into a cell-enriched fraction and a fraction of reduced cell content, for performing various analyses, such as binding assays, determinations involving polynucleotide amplification and the like. Analytical systems including such devices are also disclosed.Type: GrantFiled: January 27, 1999Date of Patent: February 6, 2001Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Patent number: 5955029Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber).Type: GrantFiled: March 12, 1996Date of Patent: September 21, 1999Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Patent number: 5928880Abstract: A mesoscale sample preparation device capable of providing microvolume test samples, separated into a cell-enriched fraction and a fraction of reduced cell content, for performing various analyses, such as binding assays, determinations involving polynucleotide amplification and the like. Analytical systems including such devices are also disclosed.Type: GrantFiled: June 11, 1997Date of Patent: July 27, 1999Assignee: Trustees of the University of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka
-
Patent number: 5866345Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.Type: GrantFiled: March 5, 1997Date of Patent: February 2, 1999Assignee: The Trustees Of The University Of PennsylvaniaInventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel