Abstract: The present invention relates to a method and device for sterile packing of a substance (2) in a container (1), whereby said substance (2) can not stand the same sterilizing process as the one said container (1) is subjected to. The container (1) is sterilized without containing said substance (2) and while being enclosed in an outer package (4) so that said container (1) becomes internally and externally sterile. Thereafter, said substance (2), which has been sterilized in another way, is inserted into the container (1) without contaminating neither the container (1) nor the substance (2) during said insertion of said substance (2) into said container (1).