Abstract: Provided herein is a method for imaging RNA transferred from a sample to a substrate. The method may include placing a sample comprising cells on a substrate, transferring RNA from the sample onto the substrate to produce an RNA blot in which the RNA is immobilized on the substrate, removing the sample from the substrate, hybridizing the RNA blot with a set of oligonucleotides that hybridize to different sites in the same RNA species, and reading the blot to obtain an image showing the binding pattern of the hybridized oligonucleotides.