Abstract: The disclosure provides, at least in part, to a method for producing high titer lentiviral vectors, and for producing lentiviral particles carrying a transgene of interest and under satisfactory safety conditions. The disclosure also provides at least in part, methods of purification of such lentiviral particle, e.g., from a cell culture. The disclosure also provides a formulation to lentiviral preparations that maintain structural integrity of the viral vector during purification, storage, and gene transfer events.