Abstract: A processor for demixing a fluorescent-light input signal of a fluorescence microscope, the fluorescent-light input signal including at least two fluorescence emission responses that overlap in time, each of the at least two fluorescence emission responses being representative of an individual impulse response of a fluorophore to a fluorescence-triggering light pulse of a clocked time series of fluorescence-triggering light pulses, the processor: receiving a trigger signal comprising a time series of time markers, the trigger signal being representative of a clocking rate, at which the clocked time series of fluorescence-triggering light pulses is generated; and separating at least one fluorescence emission response from the fluorescent-light input signal.

Abstract: An image scanning microscope includes an excitation unit that generates excitation light according to an excitation modality, and an objective lens directed at a sample space to direct the excitation light and to receive the detection light. A scanning unit of the image scanning microscope is arranged along a beam path between the excitation unit and the objective lens to selectively direct the excitation light. The image scanning microscope includes a detection arrangement including a spectral encoding element to change the spatial distribution of the intensity of the detection light and an array detector. A main beam splitter of the image scanning microscope directs the excitation light into the objective lens, and directs the detection light. The image scanning microscope includes a control unit to control the excitation unit to set the excitation modality, and to determine a spatial distribution of a concentration of two different fluorophore species.

Abstract: A detection arrangement for an optical scanning microscope includes a first beam path comprising a first array detector, and an acousto-optical device configured to receive descanned detection light, and to direct a first part of the detection light into the first beam path. The first part of the detection light includes at least one selected wavelength range determined by at least one frequency of acoustic waves generated by a transducer of the acousto-optical device. The detection arrangement further includes a controller configured to control the transducer of the acousto-optical device for determining the at least one selected wavelength range.

Abstract: A detection arrangement for a microscope includes a beam splitter configured to split detection light into a first part and a second part, directed into a first beam path and a second beam path, respectively, a first dispersive element configured to spectrally separate the first part along a first direction, a first array detector configured to receive the spectrally separated first part, a second dispersive element configured to spectrally separate the second part along a second direction, and a second array detector configured to receive the spectrally separated second part. The beam splitter, the first dispersive element, and the second dispersive element are configured such that a first reference direction corresponding to the first direction imaged back to a plane arranged before the beam splitter and a second reference direction corresponding to the second direction imaged back to the plane are different from each other.

Abstract: A computer-implemented method for computing an estimate of a first signal of a plurality of signals is provided. The plurality of signals is contained in a digital color input image. Each signal has a different ground truth spectrum. The digital color input image includes a plurality of pixels. The method includes extracting a subset from the plurality of pixels, extracting from the subset by spectral unmixing a preliminary estimate of the first signal as a first unmixed signal, and a preliminary estimate of at least one further signal of the plurality of signals as at least one further unmixed signal, computing from the subset an estimate of a dependency of the first unmixed signal on the at least one further signal as a crosstalk quantity for the subset, and removing the crosstalk quantity from the first unmixed signal to obtain the estimate of the first signal for the subset.

Abstract: A multi-core processor system includes a plurality of processors, a first global buffer, and a second global buffer. The multi-core processor system distributes a kernel to the plurality of processors for concurrently executing a plurality of threads of the kernel by each processor. While each thread of the kernel is concurrently executed, processing steps defined within the kernel are iteratively performed. The processing steps include (i) computing a forward transform of intermediary input image data, received from the first global buffer, and providing the forward transformed data to the shared memory, (ii) processing the forward transformed data to provide the processed data to the shared memory, (iii) computing a backward transform of the processed data and providing the backward transformed data to the second global buffer, and (iv) synchronizing the first global buffer between the plurality of processors before the first global buffer provides the intermediary input image data.

Abstract: A vertical impregnation bath for a pultrusion line includes a vertically-oriented reservoir having an inlet adjacent a top end of the reservoir and a terminal area at a bottom end of the reservoir, wherein the inlet is larger than the terminal area of the reservoir and the inlet is above the terminal area of the reservoir, the reservoir further having an outlet adjacent to the terminal area.

Abstract: The present invention relates to a method for generating and/or obtaining specific binding moieties against intrinsically disordered proteins (IDPs) and/or intrinsically disordered protein domains which tend to be immunologically inert and lack immunogenicity in animals, in particular in mammals. The present invention also relates to such specific binding moieties, in particular to antibodies and/or to antigen binding fragments thereof, specifically binding to structurally disordered and/or intrinsically disordered sequences, in particular to Pro/Ala-rich sequences (PAS). These binding moieties, antibodies, antigen binding fragments are first in class since they bind to/recognize disordered peptides or polypeptide fragments as also comprised in such “intrinsically disordered proteins”, in particular PAS polypeptides. The inventive binding moieties, antibodies, antigen binding fragments are, without being limiting, particularly useful in diagnostic settings as well as research tools.

Abstract: A method for scanning a sample in microscopy includes generating at least three illumination spots in order to form a spot pattern that contains at least two illumination spots having a first wavelength and an illumination spot having a second wavelength that differs from the first wavelength. At least one specified region of the sample is scanned by moving the spot pattern formed by the illumination spots along a first direction for generating scan lines, which are each associated with the illumination spots of the spot pattern, and by moving the spot pattern formed by the illumination spots along a second direction for generating scan lines respectively after the scan lines.

Abstract: Provided is a modified B-type natriuretic peptide (BNP) comprising a covalently attached polymer comprising amino acids, where the polymer inhibits degradation and/or elimination of the BNP in a subject, and where the modified BNP retains vasorelaxant activity. Nucleic acid molecules encoding the above-described modified BNP, as are vectors comprising the nucleic acid molecules, and cells comprising the vector. Methods of treating a subject suffering from or diagnosed with a disease, disorder, or medical condition that can be treated with a natriuretic, diuretic or vasorelaxant is also provided. The methods comprise administering the above modified BNP to the subject. Further provided is method of preparing the above-described modified BNP.

Abstract: The disclosure provides a modified protein that is a combination of (i) an L-asparaginase and (ii) one or more (poly)peptide(s), wherein the (poly)peptide consists solely of proline and alanine amino acid residues, and methods of preparation and use thereof.

Abstract: A method for estimating a stimulated emission depletion microscopy (STED) resolution includes generating a first frame representing a reference image from a field-of-view, the reference image having a predetermined reference resolution, and generating at least one second frame representing a STED image from the same field-of-view, the STED image having the STED resolution to be estimated. The at least one second frame is blurred by applying a convolution kernel with at least one fit parameter to the second frame. An optimal value of the at least one fit parameter of the convolution kernel is determined for which a difference between the first frame and the blurred at least one second frame is minimized. The STED resolution is estimated based on the optimal value of the at least one fit parameter and the predetermined reference resolution.

Abstract: The disclosure provides a modified protein that is a combination of (i) an L-asparaginase and (ii) one or more (poly)peptide(s), wherein the (poly)peptide consists solely of proline and alanine amino acid residues, and methods of preparation and use thereof.

Abstract: A method for scanning a sample includes generating at least two illumination points in order to form a point pattern, wherein the point pattern has a settable number of illumination points. At least one freely selectable parameter for defining the point pattern is preset or is set. At least one predefined region of the sample is scanned by moving the point pattern defined by the freely selectable parameter along a first direction such that scan lines assigned to the illumination points of the point pattern are generated, and along a second direction such that further scan lines are generated in each case following the scan lines. The movement of the point pattern in the second direction is carried out in scan steps of identical size or at a constant speed. The illumination points of the point pattern are arranged on a line along the second direction.

Abstract: A digital image processing apparatus for computing a baseline estimate of a digital input image is provided. The digital image processing apparatus is configured to obtain a digital intermediate image by downsampling the digital input image by a predetermined downsampling factor, and compute the baseline estimate based on the digital intermediate image.

Abstract: A fluorescence scanning microscope includes excitation and de-excitation light sources, which are designed to generate an excitation and a de-excitation light distribution, respectively. An illumination unit combines the light distributions to form a light distribution scanning over multiple illumination target points of a sample in such a way that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another. A detector detects fluorescence photons emitted from the respective illumination target point as a function of their arrival times. A processor evaluates the fluorescence photons with respect to the arrival times, generates a first pixel and a second pixel based thereon, assembles the first and second pixels to form first and second sample images, respectively, and, by means of the two sample images, determines a spatial offset between the intensity maximum and the intensity minimum.

Abstract: A processor for demixing a fluorescent-light input signal of a fluorescence microscope, the fluorescent-light input signal including at least two fluorescence emission responses that overlap in time, each of the at least two fluorescence emission responses being representative of an individual impulse response of a fluorophore to a fluorescence-triggering light pulse of a clocked time series of fluorescence-triggering light pulses, the processor: receiving a trigger signal comprising a time series of time markers, the trigger signal being representative of a clocking rate, at which the clocked time series of fluorescence-triggering light pulses is generated; and separating at least one fluorescence emission response from the fluorescent-light input signal.

Abstract: A fluorescence scanning microscope includes excitation and de-excitation light sources, which are designed to generate an excitation and a de-excitation light distribution, respectively. An illumination unit combines the light distributions to form a light distribution scanning over multiple illumination target points of a sample in such a way that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another. A detector detects fluorescence photons emitted from the respective illumination target point as a function of their arrival times. A processor evaluates the fluorescence photons with respect to the arrival times, generates a first pixel and a second pixel based thereon, assembles the first and second pixels to form first and second sample images, respectively, and, by means of the two sample images, determines a spatial offset between the intensity maximum and the intensity minimum.

Abstract: A fluorescence microscope includes excitation and de-excitation light sources designed to generate excitation and de-excitation light distributions, which excite and de-excite fluorophores present in a sample, respectively. An illumination unit is designed to combine the light distributions such that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another in an illumination target point. A detector is designed to detect the fluorescence photons as a function of their arrival times. The processor is designed to evaluate the detected fluorescence photons with respect to their arrival times and, based thereon, to control a delay which a light pulse or a light modulation of the de-excitation light distribution has at a position of the illumination target point in relation to a light pulse or a light modulation of the excitation light distribution.

Abstract: The disclosure provides a modified protein that is a combination of (i) an L-asparaginase and (ii) one or more (poly)peptide(s), wherein the (poly)peptide consists solely of proline and alanine amino acid residues, and methods of preparation and use thereof.