Patents by Inventor Lars G. Ljungdahl
Lars G. Ljungdahl has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 6602700Abstract: Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a feruloyl esterase of Ruminococcus. The fourth specifically exemplified phenolic acid esterase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided. Further provided are methods for improving nutrient availability and ferulic acid availability when food or feed, or other material is treated with a phenolic acid esterase, desirably in combination with a xylanase.Type: GrantFiled: June 21, 2000Date of Patent: August 5, 2003Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Michael J. Azain, Edward T. Davies, Ashit K. Shah, David L. Blum, Irina Kataeva
-
Patent number: 6534286Abstract: The present disclosure provides methods and DNA molecules for the synthesis of heterologous proteins in the fungus Aureobasidium pullulans either intracellularly or with secretion out of the cells using a regulated xylanase promoter and for secreted protein synthesis, a signal sequence. Further described are kits containing host cells for recombinant protein production, a vector containing an XynA transcription regulatory sequence, and instructions for using the vector to transform the host cells.Type: GrantFiled: June 15, 2000Date of Patent: March 18, 2003Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl
-
Patent number: 6365390Abstract: Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a xylanase of Ruminococcus. The fourth specifically exemplified xylanase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided.Type: GrantFiled: September 3, 1999Date of Patent: April 2, 2002Assignee: University of Georgia Research Foundation, Inc.Inventors: David L. Blum, Irina Kataeva, Xin-Liang Li, Lars G. Ljungdahl
-
Patent number: 6268198Abstract: The present invention provides three fungal cellulases, their coding sequences, recombinant DNA molecules comprising the cellulase coding sequences, recombinant host cells and methods for producing same. The present cellulases are from Orpinomyces PC-2.Type: GrantFiled: October 12, 2000Date of Patent: July 31, 2001Assignee: University of Georgia Research Foundation Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Huizhong Chen
-
Patent number: 6190189Abstract: The present invention provides three fungal cellulases, their coding sequences, recombinant DNA molecules comprising the cellulase coding sequences, recombinant host cells and methods for producing same. The present cellulases are from Orpinomyces PC-2.Type: GrantFiled: April 5, 1999Date of Patent: February 20, 2001Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Huizhong Chen
-
Patent number: 6184018Abstract: Provided is a novel &bgr;-glucosidase from Orpinomyces sp. PC2, nucleotide sequences encoding the mature protein and the precursor protein, and methods for recombinant production of this &bgr;-glucosidase.Type: GrantFiled: May 6, 1999Date of Patent: February 6, 2001Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Huizhong Chen, Eduardo A. Ximenes
-
Patent number: 6114158Abstract: A cDNA (1,520 bp), designated celF, consisting of an open reading frame (ORF) encoding a polypeptide (CelF) of 432 amino acids was isolated from a cDNA library of the anaerobic rumen fungus Orpinomyces PC-2 constructed in Escherichia coli. Analysis of the deduced amino acid sequence showed that starting from the N-terminus, CelF consists of a signal peptide, a cellulose binding domain (CBD) followed by an extremely Asn-rich linker region which separate the CBD and the catalytic domains. The latter is located at the C-terminus. The catalytic domain of CelF is highly homologous to CelA and CelC of Orpinomyces PC-2, to CelA of Neocallimastix patriciarum and also to cellobiohydrolase IIs (CBHIIs) from aerobic fungi. However, Like CelA of Neocallimastix patriciarum, CelF does not have the noncatalytic repeated peptide domain (NCRPD) found in CelA and CelC from the same organism.Type: GrantFiled: July 17, 1998Date of Patent: September 5, 2000Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Huizhong Chen, Lars G. Ljungdahl
-
Patent number: 6110720Abstract: A CDNA designated celE cloned from Orpinomyces PC-2 encodes a polypeptide (CelE) of 477 amino acids. CelE is highly homologous to CelB of Orpinomyces (72.3% identity) and Neocallimastix (67.9% identity), and like them, it has a non-catalytic repeated peptide domain (NCRPD) at the C-terminal end. The catalytic domain of CelE is homologous to glycosyl hydrolases of Family 5, found in several anaerobic bacteria. The gene of celE is devoid of introns. The recombinant proteins CelE and CelB of Orpinomyces PC-2 randomly hydrolyze carboxymethylcellulose and cello-oligosaccharides in the pattern of endoglucanases.Type: GrantFiled: July 17, 1998Date of Patent: August 29, 2000Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Huizhong Chen
-
Patent number: 6103511Abstract: The present invention provides a fungal lichenase, i.e., an endo-1,3-1,4-.beta.-D-glucanohydrolase, its coding sequence, recombinant DNA molecules comprising the lichenase coding sequences, recombinant host cells and methods for producing same. The present lichenase is from Orpinomyces PC-2.Type: GrantFiled: April 5, 1999Date of Patent: August 15, 2000Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Huizhong Chen
-
Patent number: 5824533Abstract: Xylanases having high specific activities from Orpinomyces sp. strain PC-2 are provided as well as methods for their purification. DNA sequences encoding these proteins are also provided.Type: GrantFiled: May 19, 1995Date of Patent: October 20, 1998Assignee: University of Georgia Research Foundation, Inc.Inventors: Xin-Liang Li, Lars G. Ljungdahl, Huizhong Chen
-
Patent number: 5591619Abstract: A xylanase from Aureobasidium pullulans having a high specific activity is provided as well as a signal protein for controlling excretion into cell culture medium of proteins to which it is attached. DNA encoding these proteins is also provided.Type: GrantFiled: September 30, 1994Date of Patent: January 7, 1997Assignee: University of Georgia Research Foundation, Inc.Inventors: Li Xin-Liang, Lars G. Ljungdahl
-
Patent number: 4385117Abstract: Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).Type: GrantFiled: November 2, 1981Date of Patent: May 24, 1983Assignee: University of Georgia Research Foundation, Inc.Inventors: Lars G. Ljungdahl, Laura H. Carriera
-
Patent number: 4292407Abstract: A newly discovered thermophilic anaerobe is described that was isolated in a biologically pure culture and designated Thermoanaerobacter ethanolicus ATCC 3/550. T. Ethanolicus is cultured in aqueous nutrient medium under anaerobic, thermophilic conditions and is used in a novel process for producing ethanol by subjecting carbohydrates, particularly the saccharides, to fermentation action of the new microorganism in a biologically pure culture.Type: GrantFiled: September 11, 1979Date of Patent: September 29, 1981Assignee: The United States of America as represented by the United States Department of EnergyInventors: Lars G. Ljungdahl, Jurgen K. W. Wiegel
-
Patent number: 4292406Abstract: A mixed culture system of the newly discovered microorganism Thermoanaerobacter ethanolicus ATCC31550 and the microorganism Clostridium thermocellum ATCC31549 is described. In a mixed nutrient culture medium that contains cellulose, these microorganisms have been coupled and cultivated to efficiently ferment cellulose to produce recoverable quantities of ethanol under anaerobic, thermophilic conditions.Type: GrantFiled: September 11, 1979Date of Patent: September 29, 1981Assignee: The United States of America as represented by the United States Department of EnergyInventors: Lars G. Ljungdahl, Jurgen K. W. Wiegel