Abstract: The present invention provides a method of determining the presence or amount of newly synthesised antibody in a sample in response to an immunogen by detecting the released antibodies or parts thereof in a sample containing lymphocytes which have been disrupted whereby to release the synthesised antibodies or parts thereof associated with said lymphocytes whereby to determine the presence or amount of newly synthesised antibody in said sample, methods of diagnosis using said method and kits for performing the method. The method may also be modified to allow detection of non-specific infection indicators.

Abstract: The present invention provides a method of determining the presence or amount of newly synthesized antibody in a sample in response to an immunogen by detecting the released antibodies or parts thereof in a sample containing lymphocytes which have been disrupted whereby to release the synthesized antibodies or parts thereof associated with said lymphocytes whereby to determine the presence or amount of newly synthesized antibody in said sample, methods of diagnosis using said method and kits for performing the method. The method may also be modified to allow detection of non-specific infection indicators.

Abstract: The present invention provides a method of detecting active antibody production in a blood sample by contacting, in the presence and absence of protein synthesis inhibitor, aliquots of the sample with a solid phase under conditions which permit antibody production and secretion by the lymphocytes; detecting in solution, binding of antibody to the antigen(s) on the solid phase; and comparing the antibody binding in the presence or absence of protein synthesis inhibitor, whereby to obtain a determination of the amount of active antibody secretion in response to the antigen(s); and kits for performing the method. The method may also be modified to allow detection of non-specific infection indicators.