Patents by Inventor Laszlo Varady
Laszlo Varady has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5756717Abstract: Disclosed are specifically designed binding, "protein imaged" sorbents which reversibly bind with high specificity and affinity a preselected macromolecule, specifically a protein. The sorbents define one or more cavities which have a binding surface complementary in shape to the molecular surface of the macromolecule and a plurality of positively and negatively charged chemical moieties spatially distributed in a mirror image and charge inverse of a subset of the ionizable groups on the molecular surface of the macromolecule. Also disclosed are methods of producing such sorbents, useful over a range of conditions, for both preparative and analytical chromatographic separations or for use in various types of analyses.Type: GrantFiled: May 24, 1995Date of Patent: May 26, 1998Assignees: PerSeptive Biosystems, Inc, Purdue Research FoundationInventors: Sandeep K. Paliwal, Timothy K. Nadler, Laszlo Varady, Fred E. Regnier
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Patent number: 5641539Abstract: Disclosed are chemically-produced specific binding, "molecular imaged" sorbents which reversibly bind a preselected macromolecule by spatially matched multipoint interactions between functional groups synthesized on the surface of the sorbent and functional groups on the surface of the macromolecule. Also disclosed are methods of producing such sorbents. The sorbents typically are high surface area solids comprising surface binding regions which have charged groups, metal coordinating groups, hydrophobic moieties, or various combination thereof anchored thereto and spaced in the mirror image of complementary interactive groups on a surface of the macromolecule.Type: GrantFiled: June 1, 1995Date of Patent: June 24, 1997Assignee: PerSeptive Biosystems, Inc.Inventors: Noubar B. Afeyan, Laszlo Varady, Fred Regnier
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Patent number: 5453199Abstract: Disclosed are chemically-produced specific binding, "molecular imaged" sorbents which reversibly bind a preselected macromolecule by spacially matched multipoint interactions between functional groups synthesized on the surface of the sorbent and functional groups on the surface of the macromolecule. Also disclosed are methods of producing such sorbents. The sorbents typically are high surface area solids comprising surface binding regions which have charged groups, metal coordinating groups, hydrophobic moities, or various combination thereof anchored thereto and spaced in the mirror image of complementary interactive groups on a surface of the macromolecule.Type: GrantFiled: May 20, 1994Date of Patent: September 26, 1995Assignee: PerSeptive Biosystems, Inc.Inventors: Noubar B. Afeyan, Laszlo Varady, Fred Regnier
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Patent number: 5417863Abstract: Disclosed is a method for the rapid quantitative determination of low density lipoprotein (LDL) in a biological fluid sample containing LDL, very low density lipoprotein (VLDL) and high density lipoprotein (HDL). The sample is first passed through a matrix to bind LDL to the matrix. The LDL is then eluted from the matrix differentially from VLDL and HDL. A parameter representative of the concentration of a solute exiting the matrix during the interval when LDL is eluted is then determined, wherein the parameter is proportional to the amount of the LDL in the sample. The parameter may be determined, e.g., by electronically integrating under a spectrophotometrically detected peak of eluted LDL.Type: GrantFiled: June 30, 1994Date of Patent: May 23, 1995Assignee: PerSeptive Biosystems, Inc.Inventors: Laszlo Varady, Noubar B. Afeyan, Robert Langer, Shmuel Shefer
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Patent number: 5389449Abstract: A method of derivatizing a chromatography matrix having a hydrophobic surface involves reacting the hydrophobic surface with a halosulfonating agent to produce a significant amount of halosulfone groups covalently bonded onto the surface. Sulfonamide bonds then are formed between these halosulfone groups and a group of amine functions on a polyaminated polymer to produce a pellicular anion exchange layer covalently bonded to the surface. The resulting layer is stable in the absence of cross-links between the molecules of the polyaminated polymer.Type: GrantFiled: January 5, 1993Date of Patent: February 14, 1995Assignee: PerSeptive Biosystems, Inc.Inventors: Noubar B. Afeyan, Hossein K. Hodjat, Laszlo Varady
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Patent number: 5372719Abstract: Disclosed are chemically-produced specific binding, "molecular imaged" sorbents which reversibly bind a preselected macromolecule by spacially matched multipoint interactions between functional groups synthesized on the surface of the sorbent and functional groups on the surface of the macromolecule. Also disclosed are methods of producing such sorbents. The sorbents typically are high surface area solids comprising surface binding regions which have charged groups, metal coordinating groups, hydrophobic moities, or various combination thereof anchored thereto and spaced in the mirror image of complementary interactive groups on a surface of the macromolecule.Type: GrantFiled: March 30, 1992Date of Patent: December 13, 1994Assignee: PerSeptive Biosystems, Inc.Inventors: Noubar B. Afeyan, Laszlo Varady, Fred Regnier
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Patent number: 5030352Abstract: Disclosed are pellicular materials useful in contact with proteins and as chromatography media. Rigid, inert, hydrophobic, polymeric materials are exposed to a solution comprising a solute defining plural interspersed hydrophobic and hydrophilic domains. The solute molecules adsorb onto the surface of the substrate by hydrophobic - hydrophobic interaction with their hydrophilic domains extending outwardly away from the surface into the solution. The molecules are then crosslinked in place to produce a solvent and pH resistant coating which presents a hydrophilic surface sufficient to mask hydrophobic regions therebeneath. The multidomain adsorbing compounds may comprise reactive groups which subsequently can be derivatized using conventional techniques to produce chromatography materials useful for conducting affinity, size exclusion, cationic exchange, anionic exchange, neutral hydrophobic interaction, and other forms of chromatographic separations.Type: GrantFiled: January 25, 1990Date of Patent: July 9, 1991Assignee: Purdue Research FoundationInventors: Laszlo Varady, Yan B. Yang, Steven E. Cook, Fred E. Regnier