Abstract: A knee replacement prosthesis includes a distal femoral implant, including a femoral head portion, including an outer arcuate surface, and interior posterior surface, an interior distal surface disposed at a first angle with respect to the interior posterior surface, and a first interior intermediate surface joining the interior posterior surface and the interior distal surface and being disposed at a second angle with respect to the interior posterior surface and at a third angle with respect to the interior distal surface. The distal femoral implant includes stackable femoral spacers secured to at least one of the interior posterior surface and the interior distal surface, thereby adjusting an aggregate spacing between the interior posterior surface and/or the interior distal surface and a resected surface of a distal femur of the patient. A tibial implant is also provided. Related methods of use of either implant or both are also provided.

Abstract: Provided herein are nucleic acids, recombinant adeno-associated virus (rAAV) particles, and compositions, as well as methods of use thereof for transducing medullary thyroid carcinoma cells and in treatment of disease, such as medullary thyroid carcinoma. In some aspects, the nucleic acid comprises a truncated calcitonin promoter, which is optionally encapsidated within a rAAV particle. In other aspects, the rAAV particle is a rAAV particle having a mutation in a surface-exposed amino acid, such as tyrosine, threonine, or serine, that enhances transduction of the particle into medullary thyroid carcinoma cells.

Abstract: Provided herein are methods of improving rAAV production in cells, the method comprising increasing the salt concentration in the media in which the cells are infected or transfected, cultured, or in which they produce AAV. Aspects of the disclosure relate to improved methods of rAAV production by co-infecting suspension adapted cells (e.g., suspension adapted HEK293 cells) with viruses that encode one or more AAV components for producing rAAV particles within the suspension adapted cells.

Abstract: A subsea well equipment landing body (1) includes an indicator assembly to indicate landing of the landing body or to indicate locking of the landing body. The indicator assembly has a stem bore (7), a sliding stem (11) within the stem bore that has a stem head (23) protruding beyond an external face of the landing body. A stem channel (25) in the sliding stem extends between the stem head and a stem channel mouth (27), and is in fluid communication with the outside of the landing body. A hydraulic channel (9) in the landing body is in fluid communication with the stem bore. The stem channel mouth is positionable in a communicating position, in which there is fluid communication between the hydraulic channel and the stem channel, and a non-communicating position, without fluid communication between the hydraulic channel and the stem channel.

Abstract: Provided herein are methods of improving rAAV production in cells, the method comprising increasing the salt concentration in the media in which the cells are infected or transfected, cultured, or in which they produce AAV. Aspects of the disclosure relate to improved methods of rAAV production by co-infecting suspension adapted cells (e.g., suspension adapted HEK293 cells) with viruses that encode one or more AAV components for producing rAAV particles within the suspension adapted cells.

Abstract: A subsea well equipment landing body (1) includes an indicator assembly to indicate landing of the landing body or to indicate locking of the landing body. The indicator assembly has a stem bore (7), a sliding stem (11) within the stem bore that has a stem head (23) protruding beyond an external face of the landing body. A stem channel (25) in the sliding stem extends between the stem head and a stem channel mouth (27), and is in fluid communication with the outside of the landing body. A hydraulic channel (9) in the landing body is in fluid communication with the stem bore. The stem channel mouth is positionable in a communicating position, in which there is fluid communication between the hydraulic channel and the stem channel, and a non-communicating position, without fluid communication between the hydraulic channel and the stem channel.

Abstract: A subsea well equipment landing body (1) comprising an indicator assembly to indicate landing of the landing body or to indicate locking of the landing body. The indicator assembly has a stem bore (7), a sliding stem (11) within the stem bore that has a stem head (23) protruding beyond an external face of the landing body. A stem channel (25) in the sliding stem extends between the stem head and a stem channel mouth (27), and is in fluid communication with the outside of the landing body. A hydraulic channel (9) in the landing body is in fluid communication with the stem bore. The stem channel mouth is positionable in a communicating position, in which there is fluid communication between the hydraulic channel and the stem channel, and a non-communicating position, without fluid communication between the hydraulic channel and the stem channel.

Abstract: A subsea well equipment landing body (1) comprising an indicator assembly to indicate landing of the landing body or to indicate locking of the landing body. The indicator assembly has a stem bore (7), a sliding stem (11) within the stem bore that has a stem head (23) protruding beyond an external face of the landing body. A stem channel (25) in the sliding stem extends between the stem head and a stem channel mouth (27), and is in fluid communication with the outside of the landing body. A hydraulic channel (9) in the landing body is in fluid communication with the stem bore. The stem channel mouth is positionable in a communicating position, in which there is fluid communication between the hydraulic channel and the stem channel, and a non-communicating position, without fluid communication between the hydraulic channel and the stem channel.

Abstract: Provided herein are nucleic acids, recombinant adeno-associated virus (rAAV) particles, and compositions, as well as methods of use thereof for transducing medullary thyroid carcinoma cells and in treatment of disease, such as medullary thyroid carcinoma. In some aspects, the nucleic acid comprises a truncated calcitonin promoter, which is optionally encapsidated within a rAAV particle. In other aspects, the rAAV particle is a rAAV particle having a mutation in a surface-exposed amino acid, such as tyrosine, threonine, or serine, that enhances transduction of the particle into medullary thyroid carcinoma cells.

Abstract: An implant for replacing the supporting function of an intervertebral space defined between adjacent vertebrae includes a prosthetic implant member dimensioned for insertion within a space provided by at least partial removal of the intervertebral disc. The implant member includes a first implant portion and a second implant portion. The first implant portion includes a microporous material formed to define a predetermined configuration and has a first relatively high average porosity to facilitate rapid bone ingrowth within the first implant portion. The second implant portion includes a microporous material formed to define a predetermined configuration and has a second relatively low average porosity to support and maintain the adjacent vertebrae in spaced relation during permanent fixation of the implant member within the adjacent vertebrae.

Abstract: The present invention provides for an artificial bone graft implant for use as a replacement for living bone material in surgical procedures requiring the use of bone graft material. The implant has a body configured to be implanted into a prepared site in a patient's bone tissue, with the body having a pair of opposed outer surfaces defining the body. A first and a second porous portion form the body with the first and second porous portions having pores of different sizes such that the average pore size of the first porous portion is greater than the average pore size of the second porous portion. The first porous portion of the body is formed in the shape of a core, with the core being in contact with the opposed outer surfaces of the body, and the second porous portion of the body is formed in the shape of an outer shell.