Patents by Inventor Laura C. Simmons

Laura C. Simmons has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210363178
    Abstract: The present disclosure relates to the production of multimeric polypeptides (e.g., comprising two or more subunits, each subunit comprising two or more polypeptide chains, such as a multispecific or bispecific antibody) in eukaryotic host cells. In some embodiments, provided herein are methods for producing a multimeric polypeptide in a eukaryotic host cell using a host cell that comprises a polynucleotide comprising a translation initiation sequence operably linked to an open-reading frame encoding each polypeptide of the multimeric polypeptide. Advantageously, the present disclosure demonstrates that tuning the strength of the translation initiation sequences linked to each open-reading frame allows for higher production of the multimeric polypeptide with fewer incorrectly assembled side products. The present disclosure further provides cells, methods of screening, and kits related thereto.
    Type: Application
    Filed: July 19, 2021
    Publication date: November 25, 2021
    Applicant: Genentech, Inc.
    Inventors: Laura C. SIMMONS, Noelia BLANCO TALAVAN
  • Patent number: 11091530
    Abstract: Provided herein are methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes.
    Type: Grant
    Filed: July 25, 2018
    Date of Patent: August 17, 2021
    Assignee: Genentech, Inc.
    Inventors: James Giulianotti, Dorothea E. Reilly, Kieran Aurori, Laura C. Simmons
  • Publication number: 20190085045
    Abstract: Provided herein are methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes.
    Type: Application
    Filed: July 25, 2018
    Publication date: March 21, 2019
    Applicant: Genentech, Inc.
    Inventors: James GIULIANOTTI, Dorothea E. REILLY, Kieran AURORI, Laura C. SIMMONS
  • Patent number: 10066002
    Abstract: Provided herein are methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes.
    Type: Grant
    Filed: November 5, 2015
    Date of Patent: September 4, 2018
    Assignee: GENENTECH, INC.
    Inventors: James Giulianotti, Dorothea Reilly, Kieran Aurori, Laura C. Simmons
  • Publication number: 20160159880
    Abstract: Provided herein are methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes.
    Type: Application
    Filed: November 5, 2015
    Publication date: June 9, 2016
    Applicant: GENENTECH, INC.
    Inventors: James GIULIANOTTI, Dorothea REILLY, Kieran Aurori, Laura C. Simmons
  • Publication number: 20110111408
    Abstract: The present invention relates generally to the fields of molecular biology and protein technology. More specifically, the invention concerns signal sequences for the secretion of heterologous polypeptide from bacteria. The invention also concerns recombinant polypeptides and uses thereof.
    Type: Application
    Filed: November 5, 2010
    Publication date: May 12, 2011
    Applicant: Genentech, Inc.
    Inventors: Matthew Marrichi, Dorothea E. Reilly, Laura C. Simmons
  • Patent number: 6979556
    Abstract: The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.
    Type: Grant
    Filed: December 13, 2001
    Date of Patent: December 27, 2005
    Assignee: Genentech, Inc.
    Inventors: Laura C. Simmons, Laura Klimowski, Dorothea Reilly, Daniel G. Yansura
  • Publication number: 20030073164
    Abstract: The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.
    Type: Application
    Filed: December 13, 2001
    Publication date: April 17, 2003
    Applicant: GENENTECH, INC.
    Inventors: Laura C. Simmons, Laura Klimowski, Dorothea Reilly, Daniel G. Yansura
  • Publication number: 20030017592
    Abstract: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
    Type: Application
    Filed: April 9, 2001
    Publication date: January 23, 2003
    Applicant: GENENTECH, INC.
    Inventors: Laura C. Simmons, Daniel G. Yansura
  • Patent number: 6242177
    Abstract: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
    Type: Grant
    Filed: March 1, 1995
    Date of Patent: June 5, 2001
    Assignee: Genentech, Inc.
    Inventors: Laura C. Simmons, Daniel G. Yansura
  • Patent number: 5840523
    Abstract: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
    Type: Grant
    Filed: March 1, 1995
    Date of Patent: November 24, 1998
    Assignee: Genetech, Inc.
    Inventors: Laura C. Simmons, Daniel G. Yansura
  • Patent number: 5747662
    Abstract: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
    Type: Grant
    Filed: March 1, 1995
    Date of Patent: May 5, 1998
    Assignee: Genentech, Inc.
    Inventors: Laura C. Simmons, Daniel G. Yansura