Abstract: The present invention provides methods of obtaining information relevant to monitoring and diagnosing cancer, particularly monitoring the progression or development of cancer, monitoring the response of cancer to treatment, diagnosing cancer, making a cancer prognosis, predicting the likelihood of a cancer responding to treatment and stratifying subjects having cancer. The methods involve determining the ratio of circulating CCL17 to circulating CCL22 in a sample from a subject.

Abstract: The present invention provides methods of obtaining information relevant to monitoring and diagnosing cancer, particularly monitoring the progression or development of cancer, monitoring the response of cancer to treatment, diagnosing cancer, making a cancer prognosis, predicting the likelihood of a cancer responding to treatment and stratifying subjects having cancer. The methods involve determining the ratio of circulating CCL17 to circulating CCL22 in a sample from a subject.

Abstract: There is disclosed an assay to determine the effect of an applied agent or influence or other stimulus on the sub-cellular localisation of the expressed product of a nucleotide sequence (“the nucleotide sequence under investigation”), comprising: (i) transfecting a cell population with a cloned expression vector comprising a composite nucleotide sequence encoding the nucleotide sequence under investigation and a detectable tag, arranged such that the product of the expressed composite nucleotide sequence includes the expressed detectable tag; (ii) applying the applied agent, influence or other stimulus to at least a portion of the transfected cell population; (iii) observing the sub-cellular location of the detectable tag in the transfected cell population and comparing the observed sub-cellular localisation to the sub-cellular localisation of the detectable tag in the cell population in the absence of said applied agent or influence.